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A Single Step Fast Track Methodology for In-Situ Detection of Hazardous Melamine by Using Highly Sensitive
Anila Arshad1, Raheel Akram2, Shahid Iqbal Rana3
1State Key Laboratory of Chemical Resource Engineering, Beijing Key Laboratory of Environmentally Harmful Chemical Analysis, Beijing University of Chemical Technology, Beijing 100029, China.
Abstract:
A single step fast track method is proposed to determine melamine based on strong hydrogen bonding between exocyclic amine group in melamine moiety and hydroxyl group of tannic acid (TA); acting as reducing and functionalizing agent for synthesis of gold nanoparticles (Au NPs). The proposed strategy is facile and highly time saving; as the detection of melamine with labelfree gold nanoparticles was accomplished within five minutes. The colorimetric probe exhibits high detection limit of 8 nM for melamine determination in raw milk, which is very low as compared to other capping ligands employed for Au NPs such as borohydride, citrate and 3,5-dihydroxybenzoic acid. The results show a highly sensitive linear range (0.03-2.5 μM) between absorption ratio and concentration of melamine with a correlation coefficient of 0.991. Proposed methodology is proven effective for on-site screening without using costly instrumentation.
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