Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Self-Serving Bias01:29

Self-Serving Bias

226
Self-serving bias is a cognitive phenomenon in which individuals attribute positive outcomes to internal factors such as their abilities, intelligence, or effort while attributing negative outcomes to external circumstances. This cognitive distortion helps maintain self-esteem but can also impede objective self-assessment.Theoretical Explanations of Self-Serving BiasTwo primary theories explain the self-serving bias: the cognitive explanation and the motivational explanation.The cognitive...
226
Specific Heat01:16

Specific Heat

67.3K
The specific heat capacity of a substance refers to the energy required to increase the temperature of one gram of that substance by one degree Celcius. Specific heat capacity is often represented in calories (cal), grams (g), and degrees Celsius (oC), but can also be expressed in joules (J), kilograms (kg), and Kelvin (K), among other units.
For example, increasing the temperature of one gram of water by 1°C requires one calorie of heat energy and can be written as 1 cal/g-°C, or...
67.3K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.3K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.3K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.4K
5.4K
Density, Specific Weight, Specific Gravity and Compressibility of Fluid01:27

Density, Specific Weight, Specific Gravity and Compressibility of Fluid

1.6K
Density, specific weight, specific gravity, and compressibility are fundamental properties of fluids. Density is the mass per unit volume, characterizing the mass of a fluid system. It influences buoyancy, pressure, flow dynamics, viscosity, thermal conductivity, and sound propagation. For instance, in pipeline design, accurate density measurements ensure that the pipeline can handle the fluid's mass.
Specific weight represents the weight per unit volume and is calculated by multiplying...
1.6K
Sensitivity, Specificity, and Predicted Value01:13

Sensitivity, Specificity, and Predicted Value

1.2K
In healthcare diagnostics, laboratory tests play a crucial role in identifying and diagnosing a wide range of medical conditions. However, interpreting test results is not always straightforward. An abnormal test result does not always confirm the presence of a disease, just as a normal result does not guarantee its absence. To assess the reliability of these diagnostic tools, healthcare practitioners rely on two key statistical indicators: sensitivity and specificity.
Sensitivity is the...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diastolic echocardiographic parameters identify pediatric kidney transplant recipients with structural myocardial alterations.

Pediatric nephrology (Berlin, Germany)·2026
Same author

Evidence- and Consensus-based European Guideline for Immunosuppressive Therapy After Pediatric Kidney Transplantation.

Transplantation·2026
Same author

Assessment of Tumor Microenvironment Inflammatory Biomarkers in Metastatic Renal Cell Carcinoma Treated With Immune Checkpoint Inhibitors.

Clinical genitourinary cancer·2026
Same author

Altered SIgA-targeting of gut microbiota is associated with long-term dysbiosis in pediatric solid organ transplant recipients.

Gut microbes·2026
Same author

Author Correction: Injured epithelial cell states impact kidney allograft survival after T-cell-mediated rejection.

Nature communications·2026
Same author

Demographics, longitudinal changes and outcome of high blood pressure in children and adolescents on kidney replacement therapy: 15 years of data from the ESPN/ERA Registry.

Pediatric nephrology (Berlin, Germany)·2026
Same journal

Variants to Functions to Therapeutic Strategies Toward Genomically Informed Care for Autosomal Dominant Polycystic Kidney Disease.

Journal of the American Society of Nephrology : JASN·2026
Same journal

Personalizing Cardio-Kidney-Metabolic Therapy: Closer But Not There Yet.

Journal of the American Society of Nephrology : JASN·2026
Same journal

Autosomal Dominant Tubulointerstitial Kidney Disease: My Kingdom for a Biomarker.

Journal of the American Society of Nephrology : JASN·2026
Same journal

Beyond the Margin: Improving Noninferiority Trials of Kidney Transplant Immunosuppression.

Journal of the American Society of Nephrology : JASN·2026
Same journal

Parathyroid Hormone Receptor 1 Facilitates Cyst Growth in Genetic Models of Autosomal Dominant Polycystic Kidney Disease.

Journal of the American Society of Nephrology : JASN·2026
Same journal

Alanyl-tRNA Synthetase 1 and Cyst Growth in Autosomal Dominant Polycystic Kidney Disease.

Journal of the American Society of Nephrology : JASN·2026
See all related articles

Related Experiment Video

Updated: Jan 25, 2026

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions
08:06

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions

Published on: July 2, 2020

5.0K

Podocyte-Specific Sialylation-Deficient Mice Serve as a Model for Human FSGS.

Kristina M Niculovic1, Linda Blume1, Henri Wedekind1

  • 1Institute of Clinical Biochemistry.

Journal of the American Society of Nephrology : JASN
|May 2, 2019
PubMed
Summary
This summary is machine-generated.

Loss of sialylation in podocytes causes kidney disease (FSGS) by impairing cell adhesion. Sialic acid supplementation may offer a new therapy for FSGS and other kidney disorders.

Keywords:
cell adhesionchronic kidney diseasefocal segmental glomerulosclerosisglomerular diseaseglycosylationpodocyte

More Related Videos

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice
07:55

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice

Published on: August 4, 2011

12.2K
Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies
08:52

Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies

Published on: May 26, 2023

3.0K

Related Experiment Videos

Last Updated: Jan 25, 2026

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions
08:06

Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions

Published on: July 2, 2020

5.0K
Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice
07:55

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice

Published on: August 4, 2011

12.2K
Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies
08:52

Author Spotlight: Generation of Patient-Derived Podocytes from Skin Biopsies

Published on: May 26, 2023

3.0K

Area of Science:

  • Nephrology
  • Glycobiology
  • Cell Biology

Background:

  • Steroid-resistant nephrotic syndrome (FSGS) etiology is unclear.
  • Aberrant glycosylation and sialic acid (Sia) are implicated in kidney pathology.
  • The role of sialylation in podocyte homeostasis is not well understood.

Purpose of the Study:

  • To investigate the role of sialylation in podocyte homeostasis and FSGS development.
  • To generate and characterize podocyte-specific sialylation-deficient models.

Main Methods:

  • Generated podocyte-specific sialylation-deficient mice (PCmas) targeting CMP-Sia synthetase.
  • Utilized histologic and ultrastructural analysis for phenotypic characterization.
  • Created immortalized sialylation-deficient podocytes (asialo-podocytes) using CRISPR/Cas9 for in vitro studies.

Main Results:

  • PCmas mice developed proteinuria and FSGS with podocyte injury and glomerular defects.
  • Loss of sialylation caused foot process effacement and slit diaphragm abnormalities.
  • Asialo-podocytes exhibited impaired adhesion to collagen IV, suggesting a mechanism for disease.

Conclusions:

  • Podocyte sialylation is crucial for maintaining kidney homeostasis and preventing FSGS.
  • Impaired podocyte adhesion to the glomerular basement membrane contributes to FSGS pathogenesis.
  • Sialic acid supplementation may represent a novel therapeutic strategy for FSGS and related glomerulopathies.