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Development of polymorphic microsatellite markers for tree peony Paeonia delavayi (Paeoniaceae) using ddRAD-seq data
Shao-Lin Tan1,2, Peter M Hollingsworth3, Han-Tao Qin1,2
1CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, Yunnan, China.
Molecular Biology Reports
|May 2, 2019
Summary
Researchers developed new microsatellite markers for the tree peony Paeonia delavayi using ddRAD-seq. These markers are effective for studying population genetics and genetic diversity in this species and related peonies.
Area of Science:
- Genetics
- Botany
- Conservation Biology
Background:
- Paeonia delavayi, a tree peony, requires genetic tools for population studies.
- Understanding genetic diversity is crucial for conservation efforts.
Purpose of the Study:
- Develop novel microsatellite markers for Paeonia delavayi.
- Assess the utility of these markers for population genetic analyses.
- Investigate genetic diversity and structure within P. delavayi populations.
Main Methods:
- Utilized ddRAD-seq (double digest restriction-site associated DNA sequencing) to identify microsatellite loci.
- Designed and validated microsatellite primers.
- Genotyped three P. delavayi populations using 20 polymorphic microsatellite loci.
- Performed cross-species amplification tests in Paeonia ludlowii.
Main Results:
- Identified 529 polymorphic microsatellite loci from ddRAD-seq data.
- Successfully developed and validated 20 polymorphic microsatellite markers for P. delavayi.
- Observed significant genetic variation within and among populations.
- Demonstrated cross-species utility in P. ludlowii, with some exclusive alleles identified.
Conclusions:
- ddRAD-seq is an efficient method for microsatellite marker development in non-model organisms with large genomes.
- The new markers are valuable for investigating genetic diversity, structure, and gene flow in P. delavayi.
- These markers can aid in the conservation and management of Paeonia species.
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