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Updated: Jan 25, 2026
08:43
A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Published on: December 1, 2018
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Observation of Fluorescent Proteins in Fixed Cells
Cold Spring Harbor Protocols
|May 3, 2019
Summary
This protocol details detecting green fluorescent protein variants and dsRed-labeled proteins in fixed mouse cells. It offers a reliable method for gene expression analysis and cellular identity determination using fluorescent reporters.
Area of Science:
- Biochemistry
- Molecular Biology
- Cell Biology
Background:
- Fluorescent proteins (FPs) are widely utilized as reporters in biological research.
- They enable the detection of gene expression and the identification of specific cell types.
- Accurate detection methods are crucial for advancing research in mouse models.
Purpose of the Study:
- To provide a protocol for detecting specific fluorescent proteins in fixed cells.
- To enable the identification of gene expression and cellular identities using these reporters.
Main Methods:
- The protocol involves the detection of green fluorescent protein (GFP) spectral variants.
- It also covers the detection of proteins labeled with the dsRed fusion tag.
- The method is optimized for fixed cell samples.
Main Results:
- Successful detection of various green fluorescent protein spectral variants was achieved.
- Proteins tagged with dsRed were reliably detected using this protocol.
- The protocol demonstrated efficacy in fixed cell preparations.
Conclusions:
- This protocol offers a robust method for detecting common fluorescent proteins in fixed mouse cells.
- It serves as a valuable tool for gene expression analysis and cellular identity determination.
- The described method enhances the utility of fluorescent proteins in biological studies.
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