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Laboratory Scale Production and Purification of a Therapeutic Antibody
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Antibody Purification and Storage

Jordan B Fishman, Eric A Berg

    Cold Spring Harbor Protocols
    |May 3, 2019
    PubMed

    Antibodies have become a common and necessary tool in biochemistry, cell biology, and immunology laboratories. There are many different types of antibodies and antibody fragments being used for a myriad of applications. As a result, many different purification protocols have been developed to obtain antibodies of the desired specificity and sensitivity. Here, we introduce the options for small- to large-scale antibody purification and isolation of polyclonal and monoclonal antibodies (and fragments generated from these) that target-specific proteins, as well as methods to properly purify antibodies that recognize posttranslational modifications. Optimal conditions for the long-term storage of antibodies are also discussed.

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