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Related Concept Videos

Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Related Experiment Video

Updated: Dec 25, 2025

Antibody Labeling with Fluorescent Dyes Using Magnetic Protein A and Protein G Beads
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Labeling Antibodies Using Europium.

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    This study details a method for creating lanthanide-labeled antibodies. These antibody conjugates are useful for various applications, including proximity assays and mass cytometry.

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    Area of Science:

    • Bioconjugation Chemistry
    • Immunochemistry
    • Analytical Chemistry

    Background:

    • Antibodies labeled with metal ions have diverse applications in biological research and diagnostics.
    • Current methods often require attaching a metal chelator to the antibody molecule using cross-linking chemistry.
    • The selection of metal and chelator can be tailored for specific experimental needs.

    Purpose of the Study:

    • To describe a basic procedure for generating lanthanide-antibody conjugates.
    • To provide a foundational method applicable to various metal-chelator antibody labeling techniques.

    Main Methods:

    • Antibody conjugation using standard cross-linking chemistry to attach a metal chelator.
    • Incubation of the antibody-chelator conjugate with the desired metal ion under specific concentration and pH conditions.
    • Purification and characterization of the resulting metal-labeled antibody conjugate.

    Main Results:

    • Successful creation of lanthanide-antibody conjugates.
    • Demonstration of the general applicability of the described conjugation strategy.
    • Confirmation that the basic procedure is adaptable for different metal-chelator combinations.

    Conclusions:

    • The described method provides a straightforward approach for producing metal-labeled antibodies, specifically lanthanide conjugates.
    • This technique supports the development of novel reagents for proximity assays, MRI contrast agents, and mass cytometry.
    • The foundational procedure allows for flexibility in choosing metals and chelators for diverse bioanalytical applications.