Effect of 2 episodes of cryosurgery on carcinogen-treated hamster cheek pouch

Hamster cheek pouches were treated with the topical carcinogen DMBA for 8 weeks. 2 episodes of cryosurgery, located within the area of previous carcinogen application enhanced the neoplastic process by comparison with matched controls. Comparison with previous studies suggests that the 2 episodes of cryosurgery may have a greater effect in provoking overt malignancy than a single episode of cryosurgery.