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Updated: Jan 25, 2026

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
Published on: March 24, 2014
Enhanced lateral resolution in continuous wave stimulated emission depletion microscopy using tightly focused annular
Geon Lim1, Wan-Chin Kim2, Seunghee Oh1,3
1School of Mechanical Engineering, Yonsei University, Seoul, Republic of Korea.
Abstract:
The lateral resolution of continuous wave (CW) stimulated emission depletion (STED) microscopy is enhanced about 12% by applying annular-shaped amplitude modulation to the radially polarized excitation beam. A focused annularly filtered radially polarized excitation beam provides a more condensed point spread function (PSF), which contributes to enhance effective STED resolution of CW STED microscopy. Theoretical analysis shows that the FWHM of the effective PSF on the detection plane is smaller than for conventional CW STED. Simulation shows the donut-shaped PSF of the depletion beam and confocal optics suppress undesired PSF sidelobes. Imaging experiments agree with the simulated resolution improvement.
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