Related Experiment Video
Updated: Jan 25, 2026
10:44
A Guided Materials Screening Approach for Developing Quantitative Sol-gel Derived Protein Microarrays
Published on: August 26, 2013
14.5K
Diagnostic Potential of Transferrin Glycoforms-A Lectin-Based Protein Microarray Approach.
Ana Penezić1, Martina Križakova2, Goran Miljuš1
1Institute for the Application of Nuclear Energy (INEP), Banatska 31b, 11080, Belgrade, Serbia.
Proteomics. Clinical Applications
|May 4, 2019
Summary
A new lectin-based protein microarray platform accurately detects changes in human transferrin (hTf) glycosylation. This sensitive method can identify biomarkers for aging and diseases like diabetes and colorectal cancer.
Area of Science:
- Biochemistry and Molecular Biology
- Analytical Chemistry
- Glycomics
Background:
- Alterations in human transferrin (hTf) glycosylation patterns are associated with various diseases and aging.
- Accurate and sensitive methods are needed to analyze these glycan changes.
Purpose of the Study:
- To design and optimize a lectin-based protein microarray for investigating hTf glycans in their native form.
- To validate the platform using hTf isolated from healthy individuals and patients with type 2 diabetes mellitus (T2DM) or colorectal carcinoma (CRC).
Main Methods:
- A lectin-based protein microarray platform was developed and optimized.
- hTf from healthy individuals and patients (T2DM, CRC) was analyzed.
- Method validation involved signal-to-noise ratio (>3), signal intensity (>250 AU), and hTf concentration for high sensitivity.
Main Results:
- Six out of fourteen tested lectins met the validation criteria.
- Aleuria aurantia (AAL), Triticum vulgaris (WGA), and Phaseolus vulgaris (PHA-E) lectins showed the highest potential for differentiating population groups.
- The platform demonstrated high sensitivity, analyzing as little as 75 pg of hTf.
Conclusions:
- A sensitive, high-throughput lectin-based protein microarray platform for hTf glycan analysis has been successfully formulated.
- Detected changes in hTf glycan structures can serve as potential biomarkers for aging and diseases such as T2DM and CRC.
- This platform offers a valuable tool for glycomic biomarker discovery.
Related Concept Videos
01:28The Early Endosome: Endocytosis of Transferrin
4.7K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
4.7K
02:34DNA Microarrays
20.9K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
20.9K
01:21The Resting Membrane Potential
142.1K
Overview
142.1K
00:52Potential Energy
42.4K
The energy stored by a structure and location of matter in space is called potential energy. For instance, raising a kettlebell changes its spatial location and increases its potential energy. Similarly, a stretched rubber band contains potential energy which, under certain conditions, can be converted into other forms of energy, such as kinetic energy.
Chemical bonds that form attractive forces between atoms also contain potential energy, called chemical energy. When a chemical reaction...
Chemical bonds that form attractive forces between atoms also contain potential energy, called chemical energy. When a chemical reaction...
42.4K
01:09Potential Energy
984
A conservative force, such as a gravitational or elastic force, gives the body the capacity to do work. This capacity, measured as the potential energy, depends on the body's location or “position” relative to a fixed reference position or datum. The gravitational potential energy is considered zero at the reference point. Suppose a body is located at some vertical distance above a fixed horizontal reference or datum. In that case, the weight of the body has positive gravitational potential...
984
03:02Standard Electrode Potentials
50.0K
On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
50.0K