Stokes shift microscopy by excitation and emission imaging

In this contribution, we present a new method, based on a tunable excitation laser source and a robust common path interferometer in the detection channel. Its purpose is to image spectral excitation and emission information on a monochrome complementary metal oxide semiconductor (CMOS) camera. This allows us to spatially obtain both excitation and emission spectra of the whole imaged area and create derived images such as red-green-blue (RGB), excitation and emission maxima, and Stokes shift images. Our presented method is a further development of hyperspectral imaging that usually is limited to recording spatially resolved emission spectra. Taking advantage of the full camera chip should speed up the acquisition versus line scan or pointwise hyperspectral imaging.