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Updated: Jan 25, 2026

Temperature-Controlled Assembly and Characterization of a Droplet Interface Bilayer
Published on: April 19, 2021
Probing membrane protein properties using droplet interface bilayers
Maxwell Allen-Benton1, Heather E Findlay1, Paula J Booth1
1Department of Chemistry, King's College London, London SE1 1DB, UK.
Impact Statement:
The paper presents a comprehensive review of integral membrane protein studies utilizing droplet interface bilayers. Droplet interface bilayers are a novel method of constructing artificial lipid bilayers with enhanced stability and physicochemical complexity compared to existing methods. Their unique morphology also suggests applications in the construction of synthetic biological systems and protocells. As well as serving as a guide to in vitro membrane protein functional studies using droplet interface bilayers in the literature to date, a novel in vitro study of a flippase protein in a droplet interface bilayer is presented.
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