Related Experiment Video

Updated: Jan 25, 2026

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads
07:55

Analyzing Cell Surface Adhesion Remodeling in Response to Mechanical Tension Using Magnetic Beads

Published on: March 8, 2017

8.9K

Filamentation Regulatory Pathways Control Adhesion-Dependent Surface Responses in Yeast

Jacky Chow1, Izzy Starr1, Sheida Jamalzadeh2

  • 1Department of Biological Sciences, State University of New York at the University at Buffalo, New York 14260-1300.

Genetics
|May 5, 2019
PubMed

Insights

Multiple signaling pathways control filamentous growth in yeast, impacting surface responses like mat formation and colony patterns. These pathways also influence adhesion, deterring predators and regulating colony temperature, offering insights into fungal pathogenesis.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Signaling pathways regulate biological responses through transcriptional control of target genes.
  • In yeast, filamentous growth is a key morphogenetic response controlled by multiple signaling pathways, relevant to fungal pathogens.
  • Surface responses like mat formation and colony patterning are adhesion-dependent processes.

Purpose of the Study:

  • To investigate the role of signaling pathways in regulating adhesion-dependent surface responses in yeast.
  • To identify new regulators and targets involved in yeast surface growth.
  • To explore the functional significance of cell adhesion in yeast colonies.

Main Methods:

  • Expression profiling to analyze gene expression patterns.
  • Mutant phenotype analysis to determine the function of genes and pathways.
  • Analysis of specific target genes involved in surface growth.

Main Results:

  • Major filamentous growth pathways (fMAPK, RAS, RTG, RIM101, RPD3, ELP, SNF1, PHO85) were found to regulate mat formation and colony patterning.
  • The chromatin remodeling complex, SAGA, was identified as a regulator of these surface responses.
  • RAS and RTG pathways were shown to coregulate common target genes, and SAGA regulated targets of fMAPK, RAS, and RTG pathways.
  • Surface growth-specific targets responding to environmental stresses (low oxygen, high temperature, desiccation) were identified.
  • Cell adhesion mediated by Flo11p deterred macroscopic predators and affected colony temperature regulation.

Conclusions:

  • Signaling pathways controlling filamentous growth are crucial for regulating adhesion-dependent surface responses in yeast.
  • The SAGA complex plays a significant role in regulating yeast surface growth.
  • Cell adhesion in yeast colonies provides protection against predators and influences colony microenvironment.
  • Understanding yeast surface growth and adhesion mechanisms can provide insights into fungal pathogenesis and virulence.
Keywords:
MAPK pathwaysbiofilmcomplex colonyexpression profilingfungal pathogenesisinvasive growthmatmicrobial predator–prey relationshipspseudohyphal growthsignaling networkstemperature control

More Related Videos

Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation14:46

Dendrimer-based Uneven Nanopatterns to Locally Control Surface Adhesiveness: A Method to Direct Chondrogenic Differentiation

Published on: January 20, 2018

8.2K
Surface Spreading and Immunostaining of Yeast Chromosomes12:06

Surface Spreading and Immunostaining of Yeast Chromosomes

Published on: August 9, 2015

10.5K

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
9.8K
Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.2K
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
8.4K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.6K
Drug Control Governance: Regulatory Bodies and Their Impact01:03

Drug Control Governance: Regulatory Bodies and Their Impact

Drug control governance involves the oversight and regulation of pharmaceuticals to ensure their safety and efficacy while preventing illegal drug use and trafficking. Regulatory bodies, including the US Food and Drug Administration (FDA) and the European Union's European Medicines Agency (EMA), play a central role in this process. These agencies evaluate the safety and efficacy of drugs before they can be marketed. They fund clinical trials and assess the benefits and risks associated with...
509
Response Surface Methodology01:16

Response Surface Methodology

Response Surface Methodology (RSM) is a collection of statistical and mathematical techniques used to develop, improve, and optimize processes. It is particularly valuable when many input variables or factors potentially influence a response variable.
The process of RSM involves several key steps:
638