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Updated: Jan 25, 2026
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
Published on: March 12, 2018
CRISPR-Cas9: A multifaceted therapeutic strategy for cancer treatment
Itishree Kaushik1, Sharavan Ramachandran1, Sanjay K Srivastava1
1Department of Immunotherapeutics and Biotechnology and Center for Tumor Immunology and Targeted Cancer Therapy, Texas Tech University Health Sciences Center, Abilene, TX, 79601, USA.
Abstract:
CRISPR-Cas9 is an RNA guided endonuclease that has revolutionized the ability to edit genome and introduce desired manipulations in the target genomic sequence. It is a flexible methodology and is capable of targeting multiple loci simultaneously. Owing to the fact that cancer is an amalgamation of several genetic mutations, application of CRISPR-Cas9 technology is considered as a novel strategy to combat cancer. Genetic and epigenetic modulations in cancer leads to development of resistance to conventional therapy options. Given the abundance of transcriptomic and genomic alterations in cancer, developing a strategy to decipher these alterations is critical. CRISPR-Cas9 system has proven to be a promising tool in generating cellular and animal models to mimic the mutations and understand their role in tumorigenesis. CRISPR-Cas9 is an upheaval in the field of cancer immunotherapy. Furthermore, CRISPR-Cas9 plays an important role in the development of whole genome libraries for cancer patients. This approach will help understand the diversity in genome variation among the patients and also, will provide multiple variables to scientists to investigate and improvise cancer therapy. This review will focus on the discovery of CRISPR-Cas9 system, mechanisms behind CRISPR technique and its current status as a potential tool for investigating the genomic mutations associated with all cancer types.
Insights
CRISPR-Cas9 gene editing offers a novel strategy against cancer by enabling precise genomic manipulation. This technology aids in understanding cancer mutations, developing new therapies, and advancing cancer immunotherapy.
Area of Science:
- Molecular Biology
- Genetics
- Oncology
Background:
- Cancer arises from numerous genetic and epigenetic alterations, often leading to therapy resistance.
- Understanding these complex genomic changes is crucial for developing effective cancer treatments.
- CRISPR-Cas9 technology has emerged as a powerful tool in genomic research.
Purpose of the Study:
- To review the discovery and mechanisms of the CRISPR-Cas9 system.
- To explore the application of CRISPR-Cas9 in cancer research and therapy.
- To highlight its potential in understanding cancer genomics and developing personalized treatments.
Main Methods:
- Review of scientific literature on CRISPR-Cas9 technology and its applications in cancer.
- Analysis of CRISPR-Cas9's role in generating cancer models and investigating genomic alterations.
- Discussion of CRISPR-Cas9's impact on cancer immunotherapy and whole genome libraries.
Main Results:
- CRISPR-Cas9 enables precise genome editing for studying cancer-related mutations.
- It facilitates the creation of cellular and animal models to understand tumorigenesis.
- The technology is revolutionizing cancer immunotherapy and personalized medicine approaches.
Conclusions:
- CRISPR-Cas9 is a transformative tool for investigating cancer genomics.
- Its application holds significant promise for developing novel cancer therapies and improving patient outcomes.
- Further research utilizing CRISPR-Cas9 will deepen our understanding of cancer diversity and treatment strategies.
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