A promising enzyme anchoring probe for selective ethanol sensing in beverages

Area of Science:

• Electrochemistry
• Biosensors
• Materials Science

Background:

• Development of selective and sensitive biosensors is crucial for accurate ethanol quantification.
• Existing methods for ethanol determination may lack sensitivity or require complex procedures.

Purpose of the Study:

• To design and synthesize a novel conjugated copolymer (TBeSe-co-P3CA) for enhanced electrochemical biosensing applications.
• To develop a one-step electrochemical coating method for creating an amperometric ethanol biosensor.
• To evaluate the performance of the developed biosensor for ethanol quantification.

Main Methods:

• Electrochemical polymerization of TBeSe-co-P3CA on a graphite electrode.
• Immobilization of alcohol oxidase (AOx) onto the modified electrode surface.
• Amperometric detection of ethanol with electrochemical analysis.

Main Results:

• The TBeSe-co-P3CA copolymer exhibited excellent performance for ethanol sensing.
• The developed biosensor showed a linear calibration plot over a wide concentration range (0.085–1.7 mM).
• High sensitivity (16.44 μA/mMcm²) and a low limit of detection (0.052 mM) were achieved with a short response time.

Conclusions:

• The developed amperometric biosensor demonstrates high accuracy and reliability for ethanol determination.
• The novel TBeSe-co-P3CA copolymer offers significant potential for advanced biosensing applications.
• This sensor shows promise for practical applications, including the analysis of ethanol in commercial alcoholic beverages.