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Updated: Jan 25, 2026

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis
Published on: July 11, 2025
Synthesis and immunogenicity of the Mycobacterium tuberculosis arabinomannan-CRM197 conjugate
Yunsong Chang1, Xin Meng1, Yaxin Li1
1Key Laboratory of Industrial Microbiology , Ministry of Education , College of Biotechnology , Tianjin University of Science and Technology , Tianjin 300457 , PR China . Email: mengxin@tust.edu.cn ; Email: yupeng@tust.edu.cn ; ; Tel: +86 22 60912562.
Abstract:
Lipoarabinomannan (LAM) is a major structural surface component of Mycobacterium tuberculosis. This study describes the synthesis of the well-defined lipoarabinomannan (LAM) specific dodecasaccharide-protein conjugate and immunological studies. Arabinomannan (AM) dodecasaccharide has been efficiently synthesized and covalently conjugated to carrier proteins, including cross reactive mutant (CRM197) diphtheria toxoid and bovine serum albumin (BSA) for novel neoglycoconjugates, creating a potent T-dependent conjugate vaccine. Preliminary mice immunization studies on the neoglycoconjugate revealed that it could give rise to a strong IgG antibody titer in mice at 4.0 μg dose with an aluminum phosphate adjuvant. AM-CRM197 shows potential as an excellent candidate for a new carbohydrate-based vaccine that would be capable of eliciting a protective immune response against tuberculosis.
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