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Next generation sequencing data for use in risk assessment.

B Alex Merrick1

  • 1Molecular and Genomic Toxicology Group, Biomolecular Screening Branch, Division National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, Ph: 919-541-1531, merrick@niehs.nih.gov.

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Next-generation sequencing (NGS) offers powerful tools for analyzing RNA and DNA, revolutionizing toxicology and biomarker discovery for risk assessment. These advanced technologies enable precise identification of chemical exposure effects in tissues and biofluids.

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Area of Science:

  • Toxicology and Molecular Biology
  • Biomarker Discovery
  • Genomic and Transcriptomic Analysis

Background:

  • Next-generation sequencing (NGS) has transformed RNA and DNA analysis.
  • NGS provides mechanistic insights into toxicology and aids biomarker discovery for risk assessment.
  • Technological advancements have increased NGS sensitivity and accuracy for biomarker assays.

Purpose of the Study:

  • To review NGS-based technologies for target discovery in biological samples.
  • To demonstrate the application of NGS in identifying transcriptional changes and genomic targets.
  • To suggest the utility of NGS in toxicological risk assessment strategies.

Main Methods:

  • Description of various NGS platforms and their capabilities.
  • Application of NGS for analyzing tissue, blood, and other biofluids.
  • Integration of NGS with emerging toxicological research areas (e.g., Tox21, organotypic spheroids, liquid biopsy, epigenomics).

Main Results:

  • NGS enables high-resolution identification of molecular responses to chemical exposure.
  • NGS facilitates biomarker discovery from diverse biological samples.
  • NGS platforms are being incorporated into rapid toxicological characterization strategies.

Conclusions:

  • NGS technologies are pivotal for advancing toxicological research and risk assessment.
  • NGS offers precise tools for identifying molecular targets and biomarkers.
  • The integration of NGS into new toxicological paradigms promises more efficient and accurate assessments.