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Magnetization transfer in liposome and proteoliposome samples that mimic the protein and lipid composition of myelin

Weiqi Yang1, Jae-Seung Lee1,2, Maureen Leninger1

  • 1Department of Chemistry, New York University, New York, NY, USA.

NMR in Biomedicine
|May 7, 2019
PubMed

Insights

Brain MRI magnetization transfer (MT) effects are not fully understood. This study found that cerebrosides, not proteins, significantly contribute to MT effects in myelin models, with lower exchange rates than previously assumed.

Area of Science:

  • Biophysics
  • Magnetic Resonance Imaging
  • Neuroscience

Background:

  • Magnetization transfer (MT) is utilized in brain MRI for conditions like multiple sclerosis.
  • The precise molecular origins of MT effects and the role of proteins remain unclear.

Purpose of the Study:

  • To investigate the molecular basis of MT effects in a model myelin environment.
  • To determine the relative contributions of proteins, cholesterol, cerebrosides, and sphingomyelin to MT.

Main Methods:

  • Utilized a proteoliposome model system to mimic myelin.
  • Employed double-quantum filter experiments to determine exchange parameters.
  • Analyzed contributions within 1,2-dimyristoyl-sn-glycero-3-phosphocholine bilayers.

Main Results:

  • Cerebrosides demonstrated the most significant exchange effects, surpassing those of proteins.
  • Sphingomyelin and cholesterol exhibited minimal transfer contributions.
  • Observed exchange rates were lower than typically assumed for hydroxyl (-OH) and amine (-NH) groups.

Conclusions:

  • Cerebrosides play a more dominant role in MT effects than proteins in myelin models.
  • The findings suggest a re-evaluation of assumed exchange rates in MT imaging.
  • Provides insights into the molecular underpinnings of MT in biological systems.
Keywords:
chemical exchange saturation transfermagnetization transferwhite matter diseases

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