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Updated: Jan 25, 2026
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Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells
Published on: June 19, 2019
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Stabilizing Antibody Cocktails for Mass Cytometry
Axel R Schulz1, Sabine Baumgart1, Julia Schulze1
1German Rheumatism Research Center Berlin (DRFZ), a Leibniz Institute, Berlin, Germany.
Summary
Premixed antibody cocktails for mass cytometry degrade over time. A new cryopreservation method ensures stable antibody cocktails for at least 9 months, improving data consistency in large-scale immune profiling studies.
Area of Science:
- Immunology
- Biotechnology
- Analytical Chemistry
Background:
- Mass cytometry is crucial for large-scale immune profiling, but data consistency is challenged by reagent variability.
- Standardization methods exist, yet antibody cocktail preparation remains a significant error source.
Purpose of the Study:
- To address the instability of premixed antibody cocktails for mass cytometry.
- To develop and validate a cryopreservation method for multiplexed antibody cocktails.
Main Methods:
- Tested storage stability of premixed antibody cocktails at 4°C for 4 weeks.
- Developed and evaluated a cryopreservation method for multiplexed antibody cocktails (lanthanide, palladium, platinum conjugates) stored below -80°C.
Main Results:
- Premixed antibody cocktails showed degraded staining patterns after 4 weeks at 4°C.
- Cryopreserved antibody cocktails maintained stable staining patterns for at least 9 months at below -80°C.
Conclusions:
- Cryopreservation offers a stable, economic, and flexible solution for storing multiplexed antibody cocktails.
- This method significantly enhances data consistency in large, multi-site mass cytometry studies.
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