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Updated: Jan 25, 2026

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
Published on: December 17, 2015
Epi-illumination SPIM for volumetric imaging with high spatial-temporal resolution
Bin Yang1, Xingye Chen1,2, Yina Wang1
1Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA, USA.
Abstract:
We designed an epi-illumination SPIM system that uses a single objective and has a sample interface identical to that of an inverted fluorescence microscope with no additional reflection elements. It achieves subcellular resolution and single-molecule sensitivity, and is compatible with common biological sample holders, including multi-well plates. We demonstrated multicolor fast volumetric imaging, single-molecule localization microscopy, parallel imaging of 16 cell lines and parallel recording of cellular responses to perturbations.
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