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Updated: Jan 25, 2026

Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli
Published on: March 20, 2016
Single-Copy Knock-In Loci for Defined Gene Expression in Caenorhabditis elegans
Carlos G Silva-García1, Anne Lanjuin1, Caroline Heintz1
1Department of Genetics and Complex Diseases, Harvard T. H. Chan School of Public Health, Harvard University, Boston, Massachusetts 02115.
Abstract:
We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the Caenorhabditis elegans genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introduced as a single copy, regulated by different tissue-specific promoters. The SKI LODGE system provides a fast, economical, and effective approach for generating single-copy ectopic transgenes in C. elegans.
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