Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Impact Loading on a Cantilever Beam

Impact Loading on a Cantilever Beam

The analysis of a cantilever beam with a circular cross-section subjected to impact loading at its free end illustrates the conversion of potential energy from a dropped object into kinetic energy, which is then absorbed by the beam as strain energy. This process is crucial for understanding how materials behave under dynamic loads, which is important in fields such as construction and aerospace.
When an object is dropped onto the free end of a cantilever, its potential energy due to gravity is...

Flow Sheet

Flow Sheet

Flowsheets are valuable tools in nursing documentation. They enable healthcare professionals to efficiently record and monitor various patient assessments and measurements in a consolidated format.
Here's a closer look at the examples of flowsheets commonly used by nurses:
Graphic Sheet Documentation:

Photoreceptors and Plant Responses to Light

Photoreceptors and Plant Responses to Light

Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.

Light as Energy

Light as Energy

The energy required to carry out photosynthesis is light— typically electromagnetic radiation from the sun. The range of all possible wavelengths is known as the electromagnetic spectrum.
Photons
A photon is a discrete electromagnetic particle or bundle of energy. Photons are characterized by their frequency, wavelength, and amplitude, similar to the properties of a wave. Waves with higher frequencies transmit more energy and have shorter wavelengths than longer wavelengths that transmit...

The Wave Nature of Light

The Wave Nature of Light

The nature of light has been a subject of inquiry since antiquity. In the seventeenth century, Isaac Newton performed experiments with lenses and prisms and was able to demonstrate that white light consists of the individual colors of the rainbow combined together. Newton explained his optics findings in terms of a "corpuscular" view of light, in which light was composed of streams of extremely tiny particles traveling at high speeds according to Newton's laws of motion.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Profiling Protein Aggregate Size Using Single-Molecule Array Technology.

Analytical chemistry·2026

Same author

Divergent toxicity mechanisms of amyloid-beta aggregates arising from a single aggregation reaction.

Cell reports·2026

Same author

PD-1 signaling and PD-1 blockade-mediated tumor control are established at microvillar T cell contacts.

Science immunology·2026

Same author

Single-molecule detection methods to study alpha-synuclein aggregation in postmortem Parkinson's disease brains.

Cell reports methods·2026

Same author

A Correlative SICM-OPM Platform for Surface and Volumetric Imaging in Live Cells.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Cortisol-resistant CAR-NK cells overcome steroid-induced immunosuppression in lung cancer.

Signal transduction and targeted therapy·2026

Search research articles

Related Experiment Video

Updated: Jan 25, 2026

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid

Published on: December 2, 2022

Sensitive light-sheet microscopy in multiwell plates using an AFM cantilever.

Aleks Ponjavic^1,2,3, Yu Ye^1,4, Ernest Laue²

¹Department of Chemistry, University of Cambridge, Cambridge, United Kingdom.

Biomedical Optics Express

|May 9, 2019

Summary

We developed a novel microscope for single-molecule imaging in standard 96-well plates. This cost-effective socSPIM (single-objective cantilever selective plane illumination microscopy) enables high-throughput cellular studies.

More Related Videos

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Published on: September 28, 2022

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Published on: February 27, 2014

Related Experiment Videos

Last Updated: Jan 25, 2026

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid

Atomic Force Microscopy Cantilever-Based Nanoindentation: Mechanical Property Measurements at the Nanoscale in Air and Fluid

Published on: December 2, 2022

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy

Published on: September 28, 2022

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Published on: February 27, 2014

Area of Science:

Biophysics
Microscopy
Cell Biology

Background:

Light sheet microscopy is powerful but often complex and expensive.
Existing designs require specialized sample chambers, hindering use with standard cell cultures.

Purpose of the Study:

To develop a cost-effective and versatile light sheet microscope compatible with standard 96-well plates.
To enable high-throughput single-molecule fluorescence imaging in biological samples.

Main Methods:

Developed single-objective cantilever selective plane illumination microscopy (socSPIM).
Introduced a light sheet through the objective lens using an AFM tip.
Utilized socSPIM for 3D imaging of nuclear pore complexes, live cell imaging of lysosomes, and super-resolution imaging of T-cell membranes.

Main Results:

Demonstrated 3D imaging of nuclear pore complexes.
Achieved live whole-cell 3D imaging of lysosomes.
Performed super-resolution imaging of the T-cell membrane.
Enabled super-resolution reflected light-sheet microscopy by PAINT in 96-well plates.

Conclusions:

socSPIM overcomes limitations of traditional light sheet microscopy.
The minimal footprint of the cantilever allows for imaging in standard 96-well plates.
This technique facilitates high-throughput single-molecule imaging and super-resolution microscopy in biological research.