Improving resolution of second harmonic generation microscopy via scanning structured illumination
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Updated: Jan 25, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
Chia-Hua Yeh1, Cheng-Zn Tan1, Ching-Hsiao Arthur Cheng2
1Department of Optics and Photonics, National Central University, 300 Jhongda Rd., Jhongli City, Taoyuan County 32001, Taiwan.
Abstract:
Second harmonic generation microscopy (SHGM) is a well-known technique for examining the noncentrosymmetric structures in biomedical research. However, without real-state transitions, fluorescence-based superresolution methods cannot be applied. To improve the resolution, fringe-scanning SHGM (FS-SHGM), which combines SHGM with structured illumination based on point-scanning, is introduced in this paper. The scanning path was modulated to generate illumination patterns. For the coherent parts of SHG signals, a mathematical model of image formation and reconstruction was established. Both simulations and experiments showed a resolution improvement factor of ~1.4 in the lateral and 1.56 in the axial directions for chicken tendons and mouse skin.
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