Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Harmonic Mean01:09

Harmonic Mean

3.7K
The arithmetic mean is usually skewed towards the larger values in the data set. Therefore, to avoid this inherent bias towards smaller values, the harmonic mean is used.
Take the example of the speed of a car, which is the measure of the rate of distance traveled. If the vehicle traverses the same distance back-and-forth, its average speed equals the total distance traveled divided by the total time taken. However, if the car moves with varying speeds, then the arithmetic mean is more skewed...
3.7K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.4K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.4K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Energy in Simple Harmonic Motion01:23

Energy in Simple Harmonic Motion

12.6K
To determine the energy of a simple harmonic oscillator, consider all the forms of energy it can have during its simple harmonic motion. According to Hooke's Law, the energy stored during the compression/stretching of a string in a simple harmonic oscillator is potential energy. As the simple harmonic oscillator has no dissipative forces, it also possesses kinetic energy. In the presence of conservative forces, both energies can interconvert during oscillation, but the total energy remains...
12.6K
Simple Harmonic Motion01:21

Simple Harmonic Motion

14.8K
Simple harmonic motion is the name given to oscillatory motion for a system where the net force can be described by Hooke's law. If the net force can be described by Hooke's law and there is no damping (by friction or other non-conservative forces), then a simple harmonic oscillator will oscillate with equal displacement on either side of the equilibrium position. To derive an equation for period and frequency, the equation of motion is used. The period of a simple harmonic oscillator is given...
14.8K
Leaky Scanning02:28

Leaky Scanning

5.7K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
Same author

Cross-Country Pilot Test of a Chair-Based Acupunch Exercise Program Among Older Adults in Long-Term Care.

Western journal of nursing research·2026
Same author

Slippage reconfiguration of trinucleotide repeat hairpins impedes resolution by human replication protein A.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Association Between Activities of Daily Living Profiles and Memory Decline in Community-Dwelling Older Adults Without Cognitive Impairment: An Observational Panel Study.

Journal of advanced nursing·2026
Same author

Innovative 3D-printed dental teaching model for root canal treatment simulation.

Journal of dental sciences·2025
Same author

Impact of Enhanced Recovery After Surgery with Neuromuscular Monitoring and Sugammadex on Healthcare Costs and Effectiveness of Recovery in Patients Following Anterior Cervical Spine Discectomy.

Journal of personalized medicine·2025

Related Experiment Video

Updated: Jan 25, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.1K

Improving resolution of second harmonic generation microscopy via scanning structured illumination.

Chia-Hua Yeh1, Cheng-Zn Tan1, Ching-Hsiao Arthur Cheng2

  • 1Department of Optics and Photonics, National Central University, 300 Jhongda Rd., Jhongli City, Taoyuan County 32001, Taiwan.

Biomedical Optics Express
|May 9, 2019
PubMed
Summary

Fringe-scanning second harmonic generation microscopy (FS-SHGM) enhances resolution for noncentrosymmetric structures. This technique improves imaging of biological tissues by ~1.4x laterally and ~1.56x axially.

More Related Videos

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

590
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.5K

Related Experiment Videos

Last Updated: Jan 25, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

26.1K
Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
09:19

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy

Published on: August 29, 2025

590
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.5K

Area of Science:

  • Biomedical Optics
  • Microscopy
  • Biophysics

Background:

  • Second harmonic generation microscopy (SHGM) visualizes noncentrosymmetric structures in biomedical research.
  • Limitations exist in applying fluorescence superresolution methods due to real-state transitions.
  • Enhanced resolution is crucial for detailed biological imaging.

Purpose of the Study:

  • Introduce fringe-scanning SHGM (FS-SHGM) to improve imaging resolution.
  • Combine SHGM with structured illumination for enhanced detail.
  • Develop a mathematical model for image reconstruction in FS-SHGM.

Main Methods:

  • Implemented fringe-scanning SHGM (FS-SHGM) by modulating scanning paths for illumination patterns.
  • Utilized structured illumination principles with point-scanning SHGM.
  • Established a mathematical model for coherent SHG signal formation and reconstruction.

Main Results:

  • Achieved a lateral resolution improvement factor of approximately 1.4.
  • Demonstrated an axial resolution improvement factor of approximately 1.56.
  • Validated results through both simulations and experimental imaging of chicken tendons and mouse skin.

Conclusions:

  • FS-SHGM effectively enhances imaging resolution compared to conventional SHGM.
  • The developed mathematical model accurately describes image formation and reconstruction.
  • FS-SHGM shows significant potential for high-resolution biomedical imaging of biological tissues.