Related Experiment Video
Updated: Jan 25, 2026
10:59
Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
Published on: February 7, 2025
1.8K
Standardization of PGC-LC-MS-based glycomics for sample specific glycotyping
Christopher Ashwood1, Brian Pratt2, Brendan X MacLean2
1Department of Molecular Sciences, Macquarie University, Sydney, NSW, Australia. nicki.packer@mq.edu.au and ARC Centre of Excellence for Nanoscale Biophotonics, Macquarie University, Sydney, NSW, Australia and Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA.
The Analyst
|May 9, 2019
Summary
This study introduces a method to normalize glycan analysis using porous graphitized carbon chromatography, enabling system-independent retention values for better glycomics discovery and quantitation.
Area of Science:
- Glycomics
- Chromatography
- Mass Spectrometry
Background:
- Porous graphitized carbon (PGC) chromatography offers high-resolution glycan separation, crucial for identifying isomers and novel structures.
- Current PGC applications in glycomics are limited by the lack of system-independent retention values for normalization.
- Technical variations in retention time and peak area hinder reproducible glycan analysis.
Purpose of the Study:
- To establish system-independent retention values for PGC-based glycan analysis.
- To develop an automated normalization method for glycomics studies.
- To create a comprehensive library of PGC-separated glycan structures.
Main Methods:
- Utilized hydrolyzed dextran as an internal standard for normalization.
- Employed Skyline software for post-acquisition data processing.
- Developed a spectral MS/MS library of a dextran ladder for automated normalization.
Main Results:
- Assigned system-independent glucose unit (GU) retention values for over 300 PGC-separated glycans.
- Created predictive models for core-fucosylation and bisecting GlcNAc modifications.
- Successfully discriminated between cell culture and tissue samples based on N-glycan intensity.
Conclusions:
- The developed normalization method enhances the reliability and automation of PGC-based glycan identification and quantitation.
- The GU retention value library and predictive models facilitate glycan structure discovery.
- This approach supports advanced glycomics research and biomarker discovery.
Related Concept Videos
02:37Standard Enthalpy of Formation
49.0K
Enthalpy changes are typically tabulated for reactions in which both the reactants and products are at the same conditions. A standard state is a commonly accepted set of conditions used as a reference point for the determination of properties under other different conditions. For chemists, the IUPAC standard state refers to materials under a pressure of 1 bar and solutions at 1 M and does not specify a temperature. Many thermochemical tables list values with a standard state of 1 atm. Because...
49.0K
03:38Measurement: Standard Units
78.8K
Every measurement provides three kinds of information: the size or magnitude of the measurement (a number), a standard of comparison for the measurement (a unit), and an indication of the uncertainty of the measurement. While the number and unit are explicitly represented when a quantity is written, the uncertainty is an aspect of the errors in the measurement results.
78.8K
03:02Standard Electrode Potentials
50.0K
On comparing the reactivity of silver and lead, it is observed that the two ionic species, Ag+ (aq) and Pb2+ (aq), show a difference in their redox reactivity towards copper: the silver ion undergoes spontaneous reduction, while the lead ion does not. This relative redox activity can be easily quantified in electrochemical cells by a property called cell potential. This property is commonly known as cell voltage in electrochemistry, and it is a measure of the energy which accompanies the charge...
50.0K
02:49Calculating Standard Free Energy Changes
24.8K
The free energy change for a reaction that occurs under the standard conditions of 1 bar pressure and at 298 K is called the standard free energy change. Since free energy is a state function, its value depends only on the conditions of the initial and final states of the system. A convenient and common approach to the calculation of free energy changes for physical and chemical reactions is by use of widely available compilations of standard state thermodynamic data. One method involves the...
24.8K
01:14Standard Solutions
2.4K
Standard solutions refer to solutions with a precisely known concentration or composition. A primary standard is a highly pure, high molar mass, stable substance that is entirely soluble in water, the most commonly used solvent in analytical chemistry. The primary standard solution can be used to standardize secondary standards, which are substances with known concentrations but are less pure and stable. Standard solutions are essential for achieving accurate and reliable results in analytical...
2.4K
01:19Standards of Care II
1.0K
Nurses bear specific legal responsibilities under several federal statutes, including:
1.0K