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Fluorometric determination of ciprofloxacin using molecularly imprinted polymer and polystyrene microparticles doped
Zhuanying Li1, Zhimeng Cui1, Yiwei Tang2,3
1College of Food Science & Project Engineering, Bohai University, Jinzhou, 121013, China.
Abstract:
The authors describe a microparticle-based system for the detection of the fluoroquinolone antibiotic ciprofloxacin. The method is using the tris(dibenzoylmethane)(1,10-phenanthroline)europium(III) luminophore in polystyrene microparticles along with a molecularly imprinted polymer (MIP) for ciprofloxacin. If ciprofloxacin is captured by the MIP, it quenches the fluorescence of the luminophores. Fluorescence drops linearly in the 0.5-100 μg L-1 ciprofloxacin concentration range, and the detection limit is 92 ng L-1. The method was applied to the analysis of fish samples to assess the analytical performance of the probe. Recoveries ranged from 85.4 to 86.6%, and relative standard deviations between 2.1 and 3.9% (for n = 5). Graphical abstract Schematic presentation of a microparticle-based probe using the tris(dibenzoylmethane)(1,10-phenanthroline)europium(III) luminophore in polystyrene particles along with a molecularly imprinted polymer for ciprofloxacin. After removal of template, carboxylic groups left in the probe can bind to ciprofloxacin through hydrogen bonds.
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