[Fluorescent probe-indicator of differences between T- and B-lymphocytes in the blood]

A new physico-chemical marker for the human peripheral blood lymphocytes was worked out. The lymphocytes were vitally stained with the fluorescent probe 3-methoxybenzanthrone and measured by microfluorometry. The blood lymphocytes population was found to be heterogeneous; this population consists of the two main groups of cells differing by the intensity of their fluorescence. By means of immunological lymphocyte fractionation it was shown that one of these cell groups was represented by T-lymphocytes, and the other one--by B-lymphocytes.