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Updated: Jan 25, 2026

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
Published on: October 19, 2013
Examining Gene Expression Patterns Through Whole-Mount In Situ Hybridization
1Department of Physiology and Developmental Biology, College of Life Sciences, Brigham Young University, Provo, UT, USA. jeff_barrow@byu.edu.
Abstract:
RNA in situ hybridization is a practical technique that allows investigators to observe temporal and spatial gene expression at the RNA level in the context of whole embryos or tissues. One powerful application of in situ hybridization is to observe the consequences of genetic, toxicologic, or environmental perturbations on gene expression or morphogenesis during development. Herein, I will review the procedure to perform nonradioactive, in situ hybridization on whole-mount mouse or chick embryos.
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