Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Thin-Layer Chromatography (TLC): Overview01:11

Thin-Layer Chromatography (TLC): Overview

4.6K
Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...
4.6K
Layers of the Epidermis01:21

Layers of the Epidermis

7.9K
The epidermis, the outermost layer of the skin, is composed of several distinct layers. From deep to superficial, the layers of the epidermis are as follows:
Stratum Basale
Stratum basale, also known as the stratum germinativum, is the deepest layer of the epidermis. It is composed of a single layer of actively dividing cells called basal cells or basal keratinocytes. These cells constantly undergo cell division to replenish the upper layers of the epidermis. Additionally, melanocytes, which...
7.9K
Affinity Chromatography01:03

Affinity Chromatography

2.9K
Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
2.9K
Chromatography: Introduction01:10

Chromatography: Introduction

7.0K
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
7.0K
Thematic Layering in GIS01:30

Thematic Layering in GIS

342
In the past, planning projects such as schools or public facilities required extensive manual effort to gather and compile data. Information such as property boundaries, soil characteristics, road networks, zoning regulations, and flood zones had to be sourced individually from courthouses, utility providers, and registry offices. Assembling these datasets into a coherent format often took several months, delaying project timelines.The introduction of Geographic Information Systems (GIS)...
342
Layers of the Heart Wall01:15

Layers of the Heart Wall

5.3K
The heart wall comprises three distinct layers: the epicardium, myocardium, and endocardium. The outermost layer, the epicardium, is the visceral layer of the serous pericardium, featuring a thin, transparent mesothelial surface and an inner layer of areolar connective tissue with fat deposits that increase with age.
The myocardium, the thickest layer, consists of cardiac muscle cells interconnected by intercalated discs and crisscrossing connective tissue fibers. These muscle fibers contract...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synergistic Potential of Berberine and Metformin: Unlocking Enhanced Anti-Diabetic Efficacy.

Chemical biology & drug design·2026
Same author

Enantioseparation by Thin-Layer Chromatography.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Chromatography in Preparative/Industrial Scale Separation of Enantiomeric Mixtures.

Biomedical chromatography : BMC·2025
Same author

Integration of transcriptomics and metabolomics data revealed role of insulin resistant SNW1 gene in the pathophysiology of gestational diabetes.

Scientific reports·2025
Same author

Design, Synthesis and Application of 1,4-disubstituted 1,2,3-triazole Based Chemosensors: A Promising Avenue.

Chemical record (New York, N.Y.)·2024
Same author

Genetic and epigenetic regulation of non-coding RNAs: Implications in cancer metastasis, stemness and drug resistance.

Pathology, research and practice·2024
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jan 25, 2026

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC
13:02

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC

Published on: March 18, 2011

37.9K

Enantioseparation by Thin-Layer Chromatography.

Rituraj Dubey1,2, Ravi Bhushan3

  • 1Department of Chemistry, Badri Narayan Mukteshwar College Barhiya, Munger University, Bihar, India.

Methods in Molecular Biology (Clifton, N.J.)
|May 10, 2019
PubMed
Summary
This summary is machine-generated.

Chiral thin-layer chromatography (TLC) offers a fast, simple alternative to high-performance liquid chromatography for separating enantiomers. Various methods using chiral selectors in TLC plates or mobile phases demonstrate effective enantioseparation of amino acids and drugs.

Keywords:
Chiral selectorEnantiomer separationImpregnationLigand-exchangeThin-layer chromatography

More Related Videos

Author Spotlight: Discovering New Biopesticides from Bioactive Soil Microbe-Derived Natural Products
04:52

Author Spotlight: Discovering New Biopesticides from Bioactive Soil Microbe-Derived Natural Products

Published on: July 26, 2024

2.3K
Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
07:42

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

Published on: April 16, 2021

9.2K

Related Experiment Videos

Last Updated: Jan 25, 2026

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC
13:02

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC

Published on: March 18, 2011

37.9K
Author Spotlight: Discovering New Biopesticides from Bioactive Soil Microbe-Derived Natural Products
04:52

Author Spotlight: Discovering New Biopesticides from Bioactive Soil Microbe-Derived Natural Products

Published on: July 26, 2024

2.3K
Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
07:42

Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography

Published on: April 16, 2021

9.2K

Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Chiral Separations

Background:

  • High-performance liquid chromatography (HPLC) is the primary method for enantioseparations.
  • Chiral thin-layer chromatography (TLC) presents a viable alternative for rapid analyses requiring simpler equipment.

Purpose of the Study:

  • To explore and describe various chiral TLC approaches for separating enantiomers.
  • To demonstrate the efficacy of these methods using amino acids and basic drugs as examples.

Main Methods:

  • Impregnation of TLC adsorbents with chiral selectors (e.g., (-)-quinine, L-amino acids).
  • Preparation of custom TLC plates and use of pre-coated plates.
  • Addition of chiral selectors directly to the mobile phase or as copper metal complexes.
  • Separation of DL-selenomethionine and β-adrenergic drugs.

Main Results:

  • Successful enantioseparation of amino acids and basic drugs using different chiral TLC techniques.
  • Demonstration of multiple strategies for incorporating chiral selectors into the TLC process.
  • Validation of chiral TLC as a practical method for enantiomeric analysis.

Conclusions:

  • Chiral TLC provides an accessible and efficient platform for enantioseparation.
  • The described methods offer flexibility in chiral selector application for diverse analytes.
  • Chiral TLC is a valuable technique complementing HPLC for specific analytical needs.