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Chiral Mobile-Phase Additives in HPLC Enantioseparations
Lushan Yu1, Shengjia Wang1, Su Zeng2
1Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, People's Republic of China.
Methods in Molecular Biology (Clifton, N.J.)
|May 10, 2019
Summary
Chiral mobile-phase additive (CMPA) High-performance liquid chromatography (HPLC) directly separates chiral drug enantiomers. This method uses chiral selectors in the mobile phase with an achiral stationary phase for effective enantioseparation.
Area of Science:
- Analytical Chemistry
- Chromatography
- Organic Chemistry
Background:
- High-performance liquid chromatography (HPLC) is crucial for separating chiral drugs.
- Direct enantioseparation of chiral chemical entities is essential in pharmaceutical analysis.
- The chiral mobile-phase additive (CMPA) technique offers a direct approach to enantioseparation.
Purpose of the Study:
- To describe the application of the CMPA technique in HPLC for chiral drug separation.
- To highlight the mechanism of enantioseparation using CMPA.
- To review common chiral selectors employed in CMPA methods.
Main Methods:
- Utilizing chiral selectors dissolved in the mobile phase.
- Employing an achiral stationary phase in HPLC.
- Analyzing the formation of transient diastereomeric complexes between selectors and enantiomers.
Main Results:
- CMPA enables direct enantioseparation by forming differential transient complexes.
- The differing formation constants and distribution of complexes lead to separation.
- Successful application demonstrated with chiral ligand exchangers, macrocyclic antibiotics, and cyclodextrins.
Conclusions:
- The CMPA technique is a valuable HPLC method for direct chiral drug enantioseparation.
- The choice of chiral selector is critical for effective separation.
- This method provides a versatile approach for analyzing chiral compounds.
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