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Updated: Jan 25, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Application of Sub-2 Micron Particle Silica Hydride Derivatized with Vancomycin for Chiral Separations by Nano-Liquid
Chiara Fanali1, Salvatore Fanali2
1Department of Medicine, University Campus Bio-Medico of Rome, Rome, Italy. c.fanali@unicampus.it.
Abstract:
1.8 μm Silica hydride particles have been derivatized with vancomycin and applied to the enantioseparation of some racemic herbicides and nonsteroidal anti-inflammatory drugs (NSAIDs) by nano-liquid chromatography. The chiral stationary phase (CSP) was packed for only 11 cm and the enantiomers were separated utilizing a laboratory-assembled instrumentation. The new CSP was very effective for the separation of the above mentioned acidic compounds, while poor resolutions were obtained for basic compounds. Mixtures of acetate buffer with methanol or acetonitrile allowed the chiral resolution of all compounds. Fast chiral separation of a NSAIDs-related compound can be achieved in less than 60 s.
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