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Related Concept Videos

Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.1K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.1K
Electrophoresis: Overview01:20

Electrophoresis: Overview

3.5K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
3.5K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

911
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
911
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

1.8K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
1.8K
Chromatography: Introduction01:10

Chromatography: Introduction

6.7K
Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
6.7K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.2K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
3.2K

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Updated: Jan 10, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
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Chiral Separations by Using Capillary Electrochromatography: An Overview.

Chiara Fanali1, Susanna Della Posta2, Giovanni D'Orazio3

  • 1Department of Science and Technology for Humans and the Environment, University Campus Bio-Medico of Rome, Rome, Italy. c.fanali@unicampus.it.

Methods in Molecular Biology (Clifton, N.J.)
|November 22, 2025
PubMed
Summary
This summary is machine-generated.

Capillary electrochromatography (CEC) offers green, efficient separations by merging electrophoresis and chromatography. This review highlights CEC

Keywords:
CECCapillary electrochromatographyChiral selectorsEnantiomersMonolithic columnsOpen-tubular capillary columnsPacked-capillary columns

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Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary electrochromatography (CEC) is a miniaturized separation technique.
  • It combines high separation efficiency from capillary zone electrophoresis and high selectivity from liquid chromatography.
  • CEC is considered a green analytical technique due to low mobile phase consumption.

Purpose of the Study:

  • To summarize the main features of capillary electrochromatography.
  • To present the potential of CEC for enantiomeric separations.
  • To review chiral stationary phases and applications in CEC from 2020-2024.

Main Methods:

  • Overview of capillary electrochromatography principles.
  • Discussion of chiral stationary phases for enantioseparation.
  • Literature review of CEC applications (2020-2024).

Main Results:

  • CEC successfully separates diverse compounds like peptides, proteins, drugs, and enantiomers.
  • Various chiral stationary phases have been developed and employed in CEC.
  • Recent literature (2020-2024) shows diverse applications of CEC in enantiomeric separations.

Conclusions:

  • Capillary electrochromatography is a versatile and green separation technique.
  • CEC shows significant potential for chiral separations using advanced stationary phases.
  • Recent applications demonstrate the continued relevance and development of CEC.