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Updated: Jan 25, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
Published on: January 16, 2017
Application of Dual-Cyclodextrin Systems in Capillary Electrophoresis Enantioseparations
Anne-Catherine Servais1, Marianne Fillet2
1Laboratory for the Analysis of Medicines, Department of Pharmaceutical Sciences, CIRM, Quartier Hôpital, University of Liège, Liège, Belgium.
Abstract:
The enantioseparation of acidic and neutral compounds can be successfully achieved in capillary electrophoresis (CE) using dual-cyclodextrin (CD) systems. This chapter describes how to separate the enantiomers of acidic or neutral substances using dual-CD systems made up of a negatively charged CD derivative, i.e., sulfobutyl-β-CD or carboxymethyl-β-CD, in combination with a neutral one, namely heptakis(2,3,6-tri-O-methyl)-β-CD. An acidic compound (carprofen) and a weakly acidic drug (pentobarbital) were selected as model compounds.
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