Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chloramphenicol resistance cloning vector based on pUC9.

N B Quigley, P R Reeves

    Plasmid
    |January 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Sequence analysis of four Shigella boydii O-antigen loci: implication for Escherichia coli and Shigella relationships.

    Infection and immunity·2001
    Same author

    When does a clone deserve a name? A perspective on bacterial species based on population genetics.

    Trends in microbiology·2001
    Same author

    Molecular evolution of large virulence plasmid in Shigella clones and enteroinvasive Escherichia coli.

    Infection and immunity·2001
    Same author

    Sequence of the E. coli O104 antigen gene cluster and identification of O104 specific genes.

    Gene·2001
    Same author

    Population genetics of Escherichia coli in a natural population of native Australian rats.

    Environmental microbiology·2001
    Same author

    Molecular characterization of Streptococcus pneumoniae type 4, 6B, 8, and 18C capsular polysaccharide gene clusters.

    Infection and immunity·2001
    Same journal

    Enhanced resistance to macrolides and tetracyclines due to cooperation among plasmid-encoded genes associated with IS26 mobile genetic elements.

    Plasmid·2026
    Same journal

    iBiT: A vector system allowing stable genome integration and scalable expression of NanoLuc fusion proteins to monitor dynamic protein-protein interactions in intact cells.

    Plasmid·2026
    Same journal

    Comparative genomics of diverse Escherichia coli O157:H7 strains to characterize plasmids, prophages, virulence and antimicrobial resistance genes.

    Plasmid·2025
    Same journal

    Megaplasmids of the enteropathogenic species Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus represent a group of novel genetic elements unrelated to other plasmids of Vibrionaceae.

    Plasmid·2025
    Same journal

    Assembly-based analysis of the infant gut microbiome reveals novel ubiquitous plasmids.

    Plasmid·2025
    Same journal

    Expression, purification, and refolding of an optimized SARS-CoV-2 receptor binding domain in E. coli.

    Plasmid·2025
    See all related articles

    A new plasmid, pPR328, offers chloramphenicol resistance for cloning DNA. The construction method can replace ampicillin resistance in other plasmids, aiding genetic engineering research.

    Area of Science:

    • Molecular Biology
    • Genetic Engineering
    • Plasmid Technology

    Background:

    • pUC vectors are widely used for DNA cloning.
    • pBR322-based plasmids often utilize ampicillin resistance markers.
    • Efficient manipulation of DNA fragments requires versatile cloning vectors.

    Purpose of the Study:

    • To construct a novel intermediate cloning vector, pPR328.
    • To provide a chloramphenicol resistance option for pUC family DNA manipulations.
    • To develop a generalizable method for replacing ampicillin resistance cassettes in pBR322-derived plasmids.

    Main Methods:

    • Construction of plasmid pPR328.
    • Utilizing a drug resistance cassette replacement strategy.
    • Cloning DNA fragments from pUC vectors into pPR328.

    Related Experiment Videos

    Main Results:

    • Successfully constructed the chloramphenicol-resistant plasmid pPR328.
    • Demonstrated pPR328's utility as an intermediate cloning vector.
    • Validated a method for replacing ampicillin resistance with other drug resistance markers.

    Conclusions:

    • Plasmid pPR328 serves as a valuable chloramphenicol-resistant cloning vector.
    • The described construction method offers a versatile approach for plasmid engineering.
    • This work facilitates broader applications in molecular cloning and genetic manipulation.