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Enhanced visualization of cell surface glycans via a hybridization chain reaction
Xiaohong Wen1, Baoyin Yuan1, Junxun Zhang1
1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, China. guoqping@126.com kmwang@hnu.edu.cn.
Abstract:
Herein, we apply a DNA hybridization chain reaction (HCR) to achieve sensitively amplified imaging of cell surface glycosylation with reduced non-natural monosaccharide units. This method is simple, efficient, sensitive, and possesses great potential to illuminate the pathways via which cell surface glycosylation regulates cell functions.
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