NanoR: A user-friendly R package to analyze and compare nanopore sequencing data

Area of Science:

• Genomics
• Bioinformatics
• Molecular Biology

Background:

• Oxford Nanopore Technologies' MinION and GridION X5 offer real-time DNA/RNA sequencing.
• MinION is popular for its portability and low cost, while GridION X5 suits large projects.
• Existing tools lack high performance for analyzing MinION and GridION X5 data.

Purpose of the Study:

• To introduce NanoR, a user-friendly R package for nanopore sequencing data visualization.
• To simplify and enhance the extraction of meaningful information from MinION and GridION X5 outputs.
• To provide a tool capable of handling and comparing data from both sequencing platforms.

Main Methods:

• Development of a cross-platform R package named NanoR.
• Implementation of functions for data visualization and analysis of nanopore sequencing data.
• Inclusion of specific features to process GridION X5 outputs and compare data from both platforms.

Main Results:

• NanoR offers a simplified approach to nanopore data visualization.
• The package effectively extracts meaningful information from MinION sequencing data.
• NanoR uniquely handles GridION X5 data and enables direct comparison between MinION and GridION X5 outputs.

Conclusions:

• NanoR addresses the need for improved tools in nanopore sequencing data analysis.
• The R package enhances the utility of MinION and GridION X5 platforms for researchers.
• NanoR is freely available, promoting wider accessibility for genomic data analysis.