Related Experiment Video
Updated: Jan 25, 2026

Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence
Published on: January 17, 2025
Bleb Needling with Mitomycin C as Adjunctive Therapy in Failing Blebs: A Retrospective Study
Chiara Del Noce1, Aldo Vagge2, Carlo Enrico Traverso1
1Eye Clinic of Genoa, Policlinic Hospital, IRCCS Ospedale Policlinico San Martino, Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DINOGMI), University of Genoa, Genoa, Italy.
Purpose:
To evaluate the effects and complications related to use of mitomycin C (MMC) as an adjunctive therapy in bleb needling.
Methods:
Retrospective review of the records of patients affected by open-angle glaucoma who underwent a bleb revision as a treatment for failed trabeculectomy. All subjects underwent surgery with a fornix-based approach to incision. Full baseline data for each patient included a comprehensive ocular and medical history, the patient's Snellen visual acuity test results, and Goldmann applanation tonometry test results. Data were reported following observations occurring at 6, 12, 18, 24, and 30 months. In addition, the number and timing of needling with or without MMC subconjunctival injections and any short- and long-term complications were observed.
Results:
We included 101 patients, 56 (55.4%) male and 45 (44.6%) female. The mean age was 69.81 ± 16.19 years (range 28-92). Statistically significant effects of needling and needling and MMC (p values <0.05) in the decrease in intraocular pressure were observed. Three (2.9%) patients presented hypotony at the last follow-up visit. No other complications were observed.
Conclusions:
Needling bleb revision augmented with the use of MMC appears to be a more useful tool in the management of failing bleb.
Related Concept Videos
Cell Motility through Blebbing
Blebbing Through the Matrix
In multicellular...
Heart Failure VI: Adjunct Therapies
Gene Therapy
Group Therapy
Inflammatory Bowel Disease III: Diagnostic Studies and Management I-Nutritional Therapy
Diagnostic studies
A colonoscopy is the definitive screening test, distinguishing ulcerative colitis from other colon diseases with similar symptoms. During a colonoscopy test, inflamed mucosa with exudate ulcerations can be observed, and biopsies are taken to determine the histologic characteristics of the...
Psychodynamic Therapy

