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RNA Stability

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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RNA Structure

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Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...

Ribosomal RNA Synthesis

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
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Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

Published on: April 8, 2017

The RNA workbench 2.0: next generation RNA data analysis.

Jörg Fallmann¹, Pavankumar Videm², Andrea Bagnacani³

¹Bioinformatics Group, Department of Computer Science; Leipzig University, Härtelstraße 16-18, D-04107 Leipzig.

Nucleic Acids Research

|May 11, 2019

Summary

This summary is machine-generated.

RNA Workbench 2.0 offers integrated tools for analyzing diverse RNA data, simplifying complex research for molecular biologists. This platform facilitates gene expression analysis and target prediction without requiring command-line expertise.

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Transcriptomic Analysis of C. elegans RNA Sequencing Data Through the Tuxedo Suite on the Galaxy Project

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

Molecular Biology
Bioinformatics
Genomics

Background:

RNA plays a critical role in gene expression regulation.
Increasing data complexity in RNA research necessitates integrated analysis tools.
Current challenges include consolidating diverse experimental data for comprehensive insights.

Purpose of the Study:

To present RNA Workbench 2.0, an updated online resource for RNA analysis.
To provide a comprehensive suite of tools and workflows for RNA data analysis.
To enable researchers to analyze RNA data without advanced computational skills.

Main Methods:

Development of an updated online resource, RNA Workbench 2.0.
Integration of diverse RNA analysis tools and workflows.
Containerized infrastructure for analysis, training, and data exploration.

Main Results:

RNA Workbench 2.0 offers a consolidated framework for RNA-related data analysis.
The platform supports analysis, data exploration, and visualization.
It provides a ready-to-use environment for hands-on training and research.

Conclusions:

RNA Workbench 2.0 enhances RNA research by integrating diverse tools and data.
The platform democratizes complex RNA analysis for a broader research community.
Accessible online resource promotes efficient gene expression and target prediction studies.