Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Initiation of Translation02:33

Initiation of Translation

38.5K
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
38.5K
Factors Affecting Protein-Drug Binding: Protein-Related Factors01:20

Factors Affecting Protein-Drug Binding: Protein-Related Factors

535
Drug binding to proteins is a key aspect of pharmacokinetics and can influence a drug's distribution, absorption, and elimination in the body. Several factors, including the drug's physiochemical properties, protein concentration, disease states, and the number of binding sites on the protein, influence this process.
The physicochemical properties of a drug play a significant role in its ability to bind to proteins. Lipophilic drugs, which dissolve in fats, oils, and lipids, can be...
535
Factors Affecting Protein-Drug Binding: Drug-Related Factors01:18

Factors Affecting Protein-Drug Binding: Drug-Related Factors

462
Drug binding to proteins is a complex phenomenon influenced by various drug-related factors, each playing a significant role in the interaction between drugs and proteins within the body.
One crucial factor in drug-protein binding is the drug's lipophilicity or its affinity for fat. More lipophilic drugs tend to have higher binding extents. For example, highly lipophilic drugs like cloxacillin exhibit substantial protein binding, with as much as 95% of the drug binding to proteins. In...
462
Factors Affecting Protein-Drug Binding: Patient-Related Factors01:29

Factors Affecting Protein-Drug Binding: Patient-Related Factors

312
Protein-drug binding, a pivotal aspect of pharmacokinetics, is subject to considerable variability influenced by an array of patient-related factors. The intricate interplay of age, individual differences, and pathological conditions significantly impact the binding dynamics and subsequent pharmacological effects.
Age stands as a key determinant in protein-drug binding. Neonates, characterized by low albumin content, experience heightened concentrations of unbound drugs such as phenytoin and...
312
Replication in Eukaryotes02:31

Replication in Eukaryotes

204.2K
Overview
204.2K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

18.7K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The nuts-and-bolts of ribosomal protein s6 kinase 1 regulation: A shared responsibility for mTOR complexes 1 and 2.

The FEBS journal·2026
Same author

GSK3β phosphorylates Six1 transcription factor and regulates its APC/C<sup>Cdh1</sup> mediated proteosomal degradation.

Cellular signalling·2024
Same author

Expanding the view of the molecular mechanisms of autophagy pathway.

Journal of cellular physiology·2022
Same author

SIX1 transcription factor: A review of cellular functions and regulatory dynamics.

International journal of biological macromolecules·2021
Same author

TGF-β signaling: A recap of SMAD-independent and SMAD-dependent pathways.

Journal of cellular physiology·2021
Same author

MYP2 locus genes: Sequence variations, genetic association studies and haplotypic association in patients with High Myopia.

International journal of biochemistry and molecular biology·2021

Related Experiment Video

Updated: Jan 25, 2026

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
10:40

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Published on: December 28, 2016

8.3K

Eukaryotic initiation factor 4E (eIF4E): A recap of the cap-binding protein.

Asiya Batool1, Sabreena Aashaq1, Khurshid I Andrabi1

  • 1Department of Biotechnology and Bioinformatics, University of Kashmir, Srinagar, Jammu and Kashmir, India.

Journal of Cellular Biochemistry
|May 11, 2019
PubMed
Summary

Eukaryotic initiation factor 4E (eIF4E) regulates protein synthesis and cellular growth. Its activation by growth factors and role in cancer genesis, particularly through phosphorylation, makes it a therapeutic target.

Keywords:
MnkeIF4EmTORtranslationtumorigenesis

More Related Videos

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

3.3K
High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

9.2K

Related Experiment Videos

Last Updated: Jan 25, 2026

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
10:40

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Published on: December 28, 2016

8.3K
Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

3.3K
High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

9.2K

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Oncology

Background:

  • Eukaryotic initiation factor 4E (eIF4E) is a key regulator of protein synthesis, binding the 5' cap of mRNA.
  • eIF4E functions as part of the eIF4F complex, crucial for mRNA recruitment to ribosomes.

Purpose of the Study:

  • To review the role of growth factor signaling in eIF4E activation and its impact on cellular growth.
  • To explore eIF4E's involvement in cancer genesis, focusing on its phosphorylation state.
  • To highlight therapeutic strategies targeting eIF4E and related pathways.

Main Methods:

  • Literature review of recent studies on eIF4E signaling and cancer.
  • Analysis of the convergence of pathways like mTOR/PI3K and Mnk/MAPK at eIF4E.
  • Examination of eIF4E's phosphorylation status in relation to cancer progression.

Main Results:

  • Growth factor signaling pathways activate eIF4E, promoting cellular growth and protein synthesis.
  • Phosphorylation of eIF4E is linked to enhanced cancer cell growth and tumorigenesis.
  • eIF4E acts as a central node for oncogenic signaling pathways.

Conclusions:

  • Targeting eIF4E and its associated signaling pathways offers a promising therapeutic strategy for cancer.
  • Understanding eIF4E's multifaceted role in translation and cancer is crucial for developing effective treatments.