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Updated: Jan 25, 2026
Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
Published on: March 8, 2013
Use of trypan blue to assess lymphatic function following trabeculectomy
Ying J Khoo1, Aulia A H Abdullah2, Dao-Yi Yu1
1Lions Eye Institute, University of Western Australia, Nedlands, Western Australia, Australia.
Importance:
To illustrate the importance of lymphatic drainage in assessing trabeculectomy bleb function using intracameral trypan blue.
Background:
To study the lymphatic drainage of trabeculectomy blebs using trypan blue, correlate with bleb function and classify them accordingly.
Design:
Prospective cross-sectional study in a tertiary care centre.
Participants:
Thirteen glaucoma patients post-trabeculectomy were studied.
Methods:
Trypan blue was injected into the anterior chamber.
Main Outcome Measures:
The duration taken for dye to stain a drainage bleb or lymphatics is recorded. The extent of the lymphatic structures were measured in clock hours. Intraocular pressure (IOP) prior to surgery was recorded.
Results:
Eight post-trabeculectomy subjects with dye stained lymphatic vessels had lower IOP (12.6 mmHg, P = .013) compared to the five with no lymphatic vessel staining (mean IOP 23.6 mmHg). Lymphatic extent was inversely related to IOP (P = .021).
Conclusions And Relevance:
Eyes with lymphatic connections to drainage blebs had lower IOP and reduced requirement for topical medications. The extent of lymphatic connection to drainage blebs is related to lower IOP.
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