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Advances in Higher Order Multiplexing Techniques in Proteomics.

Suruchi Aggarwal1,2,3, Narayan C Talukdar2,3, Amit K Yadav1

  • 1Drug Discovery Research Centre, Translational Health Science and Technology Institute , NCR Biotech Science Cluster , Third Milestone, Faridabad - Gurgaon Expressway , Faridabad , Haryana 121001 , India.

Journal of Proteome Research
|May 11, 2019
PubMed
Summary
This summary is machine-generated.

Enhanced multiplexing in proteomics improves quantitative accuracy and coverage by combining MS¹ and MS² methods. This advanced technique saves instrument time and reduces variability for robust systems biology studies.

Keywords:
SILACTMTcPILOThyperplexingiTRAQmultiplexingproteome dynamicsquantitative proteomics

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Area of Science:

  • Proteomics
  • Mass Spectrometry (MS)
  • Systems Biology

Background:

  • Quantitative proteomics is crucial for systems biology but limited by accuracy, coverage, and missing values.
  • Statistically robust findings require multiple replicates with minimal technical variation.
  • Multiplexed labeling techniques enable quantitative comparisons across multiple samples.

Purpose of the Study:

  • To review higher order multiplexing (enhanced multiplexing) in proteomics.
  • To highlight its ability to expand multiplexing capabilities of MS methods.
  • To provide an overview of its advantages, challenges, and applications.

Main Methods:

  • Combines traditional MS¹- and MS²-based quantitative proteomics.
  • Leverages multiplexed labeling strategies.
  • Discusses innovative experimental designs utilizing enhanced multiplexing.

Main Results:

  • Enhanced multiplexing saves instrument time and reduces run-to-run variability.
  • Improves quantitation accuracy and increases proteomic coverage.
  • Facilitates statistically robust biological observations.

Conclusions:

  • Higher order multiplexing offers significant advantages for quantitative proteomics.
  • It addresses key limitations in current systems biology approaches.
  • Further adoption depends on overcoming associated challenges.