Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Diode: Reverse bias01:14

Diode: Reverse bias

1.9K
A diode is reverse-biased when the positive terminal of an external voltage source is connected to the n-type material and the negative terminal to the p-type material. This configuration opposes the natural direction of current flow through the diode, effectively increasing the width of the depletion region and the barrier potential. The reverse bias condition produces a minimal leakage current, primarily due to minority charge carriers. This leakage becomes significant when the reverse...
1.9K
Reversible and Irreversible Processes01:14

Reversible and Irreversible Processes

5.6K
The thermodynamic processes can be classified into reversible and irreversible processes. The processes that can be restored to their initial state are called reversible processes. It is only possible if the process is in quasi-static equilibrium, i.e., it takes place in infinitesimally small steps, and the system remains at equilibrium However, these are ideal processes and do not occur naturally. An ideal system undergoing a reversible process is always in thermodynamic equilibrium within...
5.6K
Gene-Environment Interactions01:20

Gene-Environment Interactions

1.1K
Gene expression is a dynamic process that is significantly influenced by environmental factors. This interaction underlies the complex nature of biological development and the phenotypic differences observed among individuals, even among those with identical genetic makeups. Factors such as radiation, temperature, behavior, nutrition, and stress play pivotal roles in determining how genes are expressed. The concept of the reaction range is central to understanding this interaction. It posits...
1.1K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.6K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.6K
Sanger Sequencing01:57

Sanger Sequencing

773.9K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
773.9K
Background and Environment Affect Phenotype02:27

Background and Environment Affect Phenotype

7.5K
Although the genetic makeup of an organism plays a major role in determining the phenotype, there are also several environmental factors, such as temperature, oxygen availability, presence of mutagens, that can alter an organism’s phenotype.
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
7.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterizing parent language about home respiratory technology for children.

Journal of child health care : for professionals working with children in the hospital and community·2026
Same author

Serological response after shorter durations of therapy for syphilis of late latent or unknown duration: What does it mean?

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2026
Same author

Treponemal and Lipoidal Immunoglobulin M Tests in the Diagnosis and Management of Syphilis Among Nonpregnant Adults: A Systematic Review.

Sexually transmitted diseases·2026
Same author

Insulin Patient Assistance Programs and Protected Heath Information.

JAMA network open·2026
Same author

"Some Other Race:" An Assessment of Race and Ethnicity Survey Responses Among Latino Immigrants in Maryland.

Journal of general internal medicine·2026
Same author

Comparing Asymptomatic Versus Symptomatic Bacterial Vaginosis: Clinical Characteristics, Vaginal Microbiota, and Lactic Acid Profiles.

Sexually transmitted diseases·2026

Related Experiment Video

Updated: Jan 25, 2026

Implantation of Total Artificial Heart in Congenital Heart Disease
07:27

Implantation of Total Artificial Heart in Congenital Heart Disease

Published on: July 18, 2014

25.1K

Evaluating congenital syphilis in a reverse sequence testing environment.

May W Chen1, Ibukunoluwa C Akinboyo2,3, Paul K Sue2,4

  • 1Division of Neonatology, Department of Pediatrics, Johns Hopkins School of Medicine, Baltimore, MD, USA.

Journal of Perinatology : Official Journal of the California Perinatal Association
|May 12, 2019
PubMed
Summary
This summary is machine-generated.

Maternal reverse-sequence (RS) syphilis screening did not improve infant management for congenital syphilis (CS). In a high-risk group, RS testing did not identify additional CS cases, with discordant results often relying on clinical judgment.

More Related Videos

Author Spotlight: Advancing Syphilis Research — Innovations in Treponema pallidum Cultivation and Genetic Engineering
09:45

Author Spotlight: Advancing Syphilis Research — Innovations in Treponema pallidum Cultivation and Genetic Engineering

Published on: January 24, 2025

7.0K
A Method to Study Adaptation to Left-Right Reversed Audition
07:14

A Method to Study Adaptation to Left-Right Reversed Audition

Published on: October 29, 2018

6.9K

Related Experiment Videos

Last Updated: Jan 25, 2026

Implantation of Total Artificial Heart in Congenital Heart Disease
07:27

Implantation of Total Artificial Heart in Congenital Heart Disease

Published on: July 18, 2014

25.1K
Author Spotlight: Advancing Syphilis Research — Innovations in Treponema pallidum Cultivation and Genetic Engineering
09:45

Author Spotlight: Advancing Syphilis Research — Innovations in Treponema pallidum Cultivation and Genetic Engineering

Published on: January 24, 2025

7.0K
A Method to Study Adaptation to Left-Right Reversed Audition
07:14

A Method to Study Adaptation to Left-Right Reversed Audition

Published on: October 29, 2018

6.9K

Area of Science:

  • Infectious Diseases
  • Maternal-Fetal Medicine
  • Clinical Diagnostics

Background:

  • Congenital syphilis (CS) poses significant risks to newborns.
  • Accurate maternal syphilis screening is crucial for preventing CS.
  • Reverse-sequence (RS) testing is a diagnostic approach for syphilis.

Purpose of the Study:

  • To evaluate the impact of maternal RS syphilis screening on the management of infants at risk for CS.
  • To assess if RS testing aids in identifying additional CS cases in a high-risk population.

Main Methods:

  • Retrospective study (2011-2014) at an academic medical center.
  • Utilized RS testing including chemiluminescent immunoassay (CIA), rapid plasma reagin (RPR), and fluorescent treponemal antibody-absorption (FTA-ABS).
  • Compared clinical management and outcomes of infants born to mothers with discordant serology (CIA+/RPR-/FTA+) against guidelines.

Main Results:

  • Sixty-three infants were analyzed: 21 discordant, 16 presumed false positive, and 26 true positive.
  • Only 24% of infants in the discordant group received the recommended full evaluation.
  • No infants in the evaluated discordant group (n=8) were diagnosed with CS.

Conclusions:

  • Management of infants with discordant maternal RS serology often depends on clinical judgment.
  • RS testing in this high-risk population did not lead to the identification of additional CS cases.
  • Standardized approaches to CS prevention and infant management require further investigation.