Related Experiment Video
Updated: Jan 25, 2026

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
Published on: August 6, 2019
Multiphosphorylation and cellular localization of poly(rC) binding protein 1 during mitosis in hela cell
Xiang Ma1, Huicai Li2, Xue Cao2
1Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Life and Pharmaceutical Sciences, Hainan University, Haikou, 570228, China.
Objective:
To monitor the phosphorylation modifications and cellular localization of poly(rC)-binding protein-1 (PCBP1) during the cell cycle progression of Hela cells.
Result:
Hela cells highly synchronized at five different phases from interphase to mitosis were obtained. Using mitotic phosphoprotein-specific monoclonal antibody MPM-2, the exclusive occurrences of multiphosphorylation statuses of PCBP1 in mitosis were confirmed by a series of spots with increasing acidic pI (isoelectric point) in two rounds of 2D western blotting on the same membrane, and a visible molecular mass shift that can be eliminated by the treatment with λ phosphatase in 1D western blotting. Immnuofluorescence revealed the localization shift of PCBP1 during cell cycle, with accumulations in nucleus as a patch pattern in interphase, and a dispersive distribution without the area of the condensed chromosomes during mitosis.
Conclusions:
These observations of mitosis-specific multiphosphorylations and localization shifts of PCBP1 suggest that the versatile PCBP1 was regulatable in a phosphorylation modification- and temporospatial-dependent manner in mitotic regulatory networks.
More Related Videos
08:33Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
Published on: December 5, 2017
12:09Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells
Published on: April 20, 2017
Related Concept Videos
Mitosis and Cytokinesis
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
Mitosis and Cytokinesis
The processes of the cell cycle occur over approximately 24 hours (in typical human cells) and in two major distinguishable stages. The...
Tissue-Drug Binding: Localization of Drugs and its Significance
Drugs can bind to different tissue components, enhancing their distribution and localization. The factors influencing drug localization in tissues include the drug's lipophilicity, structural characteristics, tissue perfusion rate, and pH differences. These factors determine...
RC Circuit with Source
Due to the inherent properties of a capacitor, its voltage cannot change instantaneously. This means that immediately after the switch is closed, the capacitor's voltage remains the same as it was just before the switch was closed.
The Equilibrium Binding Constant and Binding Strength
Factors Affecting Protein-Drug Binding: Protein-Related Factors
The physicochemical properties of a drug play a significant role in its ability to bind to proteins. Lipophilic drugs, which dissolve in fats, oils, and lipids, can be...