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MiR-499 inhibited hypoxia/reoxygenation induced cardiomyocytes injury by targeting SOX6

Yujie Shi1, Yunfeng Han2, Lili Niu2

  • 1Department of Cardiology, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing, 100853, China.

Biotechnology Letters
|May 12, 2019
PubMed
Abstract

Insights

MicroRNA-499-5p protects against hypoxia/reoxygenation-induced cardiomyocyte injury by targeting SOX6. This miR-499-5p/SOX6 pathway offers a potential therapeutic strategy for acute myocardial infarction (AMI).

Area of Science:

  • Cardiovascular Biology
  • Molecular Cardiology
  • Cellular Injury Mechanisms

Background:

  • MicroRNA-499 (miR-499) is expressed in cardiomyocytes and its levels increase after acute myocardial infarction (AMI).
  • miR-499 is implicated in cardiomyocyte injury from hypoxia/reoxygenation (H/R), but its precise role is not fully understood.

Purpose of the Study:

  • To elucidate the role of miR-499-5p in H/R-induced cardiomyocyte injury.
  • To identify the molecular targets and mechanisms through which miR-499-5p exerts its protective effects.

Main Methods:

  • Investigated the effect of hypoxia and H/R on miR-499-5p expression in H9c2 cells.
  • Utilized gene expression analysis, apoptosis assays, and measurement of injury markers (LDH, MDA).
  • Examined the interaction between miR-499-5p and its target gene SOX6, including overexpression and rescue experiments.

Main Results:

  • Hypoxia and H/R decreased miR-499-5p expression and induced apoptosis in H9c2 cells.
  • SOX6 was identified as a direct target of miR-499-5p; miR-499-5p inhibited SOX6 expression.
  • miR-499-5p overexpression reduced H/R-induced cell injury, apoptosis, LDH, and MDA levels, while upregulating Bcl-2 and downregulating Bax and caspase-3. SOX6 partially reversed these effects.

Conclusions:

  • miR-499-5p protects cardiomyocytes against H/R-induced injury by targeting SOX6.
  • The miR-499-5p/SOX6 signaling pathway represents a promising therapeutic target for acute myocardial infarction.
Keywords:
CardiomyocytesHypoxia/reoxygenationSOX6miR-499

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