Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

pre-mRNA Processing02:01

pre-mRNA Processing

57.2K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
57.2K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

6.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.6K
The Proteasome02:18

The Proteasome

10.1K
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
10.1K
Molecular Chaperones and Protein Folding03:00

Molecular Chaperones and Protein Folding

19.7K
The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
The...
19.7K
Initiation of Translation02:33

Initiation of Translation

38.5K
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
38.5K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The human milk oligosaccharide 3'sialyllactose reduces low-grade inflammation and atherosclerosis development in mice.

JCI insight·2024
Same author

Publisher Correction: IL-1 and autoinflammatory disease: biology, pathogenesis and therapeutic targeting.

Nature reviews. Rheumatology·2024
Same author

NLRP3 activation in neutrophils induces lethal autoinflammation, liver inflammation, and fibrosis.

EMBO reports·2024
Same author

The discovery of NLRP3 and its function in cryopyrin-associated periodic syndromes and innate immunity.

Immunological reviews·2023
Same author

Src- and Abl-family kinases activate spleen tyrosine kinase to maximize phagocytosis and Leishmania infection.

Journal of cell science·2023
Same author

Antisense Oligonucleotide Therapy Decreases IL-1β Expression and Prolongs Survival in Mutant Nlrp3 Mice.

Journal of immunology (Baltimore, Md. : 1950)·2023
Same journal

Facial Dysmorphism and Severe Vascular Phenotype in TRNT1 Deficiency with Concomitant Antithrombin III Deficiency.

Journal of clinical immunology·2026
Same journal

Intersection Between Systemic Autoimmune Diseases, Primary Immunodeficiency and Cancer: a Field in its Infancy.

Journal of clinical immunology·2026
Same journal

Disseminated Tuberculosis and Early-Onset SLE in a Child with a Novel STAT1 Gain-of-Function Mutation: a Case Report.

Journal of clinical immunology·2026
Same journal

Humoral Immune Abnormalities in Transient Childhood Neutropenia: Insights From a 10-year Cohort Study in a Tertiary Center.

Journal of clinical immunology·2026
Same journal

Correction to: Whole Genome Sequencing in 25 Families with Suspected Inborn Errors of Immunity: Diagnostic Yield and Clinical Relevance of Genome-wide Analysis.

Journal of clinical immunology·2026
Same journal

Hypoproteinemia and Hypogammaglobulinemia Due to a Homozygous FCGRT Mutation.

Journal of clinical immunology·2026
See all related articles

Related Experiment Video

Updated: Jan 25, 2026

Tissue Determination Using the Animal Cap Transplant ACT Assay in Xenopus laevis
11:00

Tissue Determination Using the Animal Cap Transplant ACT Assay in Xenopus laevis

Published on: May 16, 2010

12.9K

CAPS and NLRP3.

Laela M Booshehri1, Hal M Hoffman2

  • 1Division of Pediatric Allergy, Immunology, and Rheumatology, Rady Children's Hospital of San Diego, University of California, San Diego, San Diego, CA, USA.

Journal of Clinical Immunology
|May 12, 2019
PubMed
Summary
This summary is machine-generated.

Cryopyrin-associated periodic syndrome (CAPS) is a rare genetic disorder causing inflammation due to NLRP3 gene mutations. Targeted therapies activating regulatory pathways have improved patient quality of life.

Keywords:
Familial cold autoinflammatory syndromeMuckle–Wells syndromecryopyrin-associated periodic syndromesinflammasomeneonatal onset multi-system inflammatory disease

More Related Videos

Large Area Substrate-Based Nanofabrication of Controllable and Customizable Gold Nanoparticles Via Capped Dewetting
05:51

Large Area Substrate-Based Nanofabrication of Controllable and Customizable Gold Nanoparticles Via Capped Dewetting

Published on: February 26, 2019

6.1K
Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
10:40

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Published on: December 28, 2016

8.3K

Related Experiment Videos

Last Updated: Jan 25, 2026

Tissue Determination Using the Animal Cap Transplant ACT Assay in Xenopus laevis
11:00

Tissue Determination Using the Animal Cap Transplant ACT Assay in Xenopus laevis

Published on: May 16, 2010

12.9K
Large Area Substrate-Based Nanofabrication of Controllable and Customizable Gold Nanoparticles Via Capped Dewetting
05:51

Large Area Substrate-Based Nanofabrication of Controllable and Customizable Gold Nanoparticles Via Capped Dewetting

Published on: February 26, 2019

6.1K
Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
10:40

Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions

Published on: December 28, 2016

8.3K

Area of Science:

  • Immunology
  • Genetics
  • Rheumatology

Background:

  • Cryopyrin-associated periodic syndrome (CAPS) is a rare inherited autoinflammatory disorder.
  • It involves systemic, cutaneous, musculoskeletal, and central nervous system inflammation.
  • Gain-of-function mutations in NLRP3 activate the cryopyrin inflammasome, leading to cytokine release and symptoms.

Purpose of the Study:

  • To review the mechanisms regulating the cryopyrin inflammasome.
  • To discuss the development of targeted therapies for CAPS.
  • To highlight improvements in patient quality of life.

Main Methods:

  • Literature review of inflammasome regulation.
  • Analysis of genetic mutations in CAPS patients.
  • Evaluation of targeted therapy outcomes.

Main Results:

  • NLRP3 mutations are central to CAPS pathogenesis.
  • Understanding inflammasome regulation is key to therapeutic development.
  • Targeted therapies have significantly improved patient outcomes.

Conclusions:

  • Targeting cryopyrin inflammasome pathways offers effective treatment for CAPS.
  • Novel therapies have transformed the management of this orphan disease.
  • Further research into regulatory mechanisms can yield more treatment options.