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Visualization of Mass Cytometry Signal Background to Enable Optimal Core Panel Customization and Signal Threshold
Amelia Au-Yeung1, Chikara Takahashi1, W Rodney Mathews1
1OMNI Biomarkers, Development Sciences, Genentech, South San Francisco, CA, USA.
Abstract:
Signal interference or overlap in mass cytometry is minimal compared to flow cytometry but must still be considered for optimal panel design and assay sensitivity. Here we describe a procedure for evaluating signal interference dynamics in the context of a 25-parameter core immunophenotyping panel. Specifically, a mass-minus-many (MMM) approach was used to assess background signals in "empty" or "blank" channels intended for further customization. Through this approach cell type-specific variability in signal background is revealed. Further panel customization can thus be performed with an understanding of cell type and channel-specific background levels to enable rational panel design and the objective delineation of gating thresholds during analysis.
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