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Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
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Quantitative Measurement of Cell-Nanoparticle Interactions Using Mass Cytometry
Andrew J Mitchell1, Angela Ivask2, Yi Ju3
1Materials Characterisation and Fabrication Platform, Department of Chemical Engineering, University of Melbourne, Melbourne, VIC, Australia. andrew.mitchell1@unimelb.edu.au.
Methods in Molecular Biology (Clifton, N.J.)
|May 12, 2019
Summary
Mass cytometry, using inductively coupled plasma mass spectrometry (ICP-MS), quantifies cellular isotope composition. This study demonstrates ICP-MS for measuring metal nanoparticle uptake by cells in suspension.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Cell Biology
Background:
- Mass cytometry, a powerful technique, typically quantifies cellular heterogeneity using antibodies labeled with stable lanthanide isotopes.
- Inductively coupled plasma mass spectrometry (ICP-MS) is the core technology enabling mass cytometry's high-resolution cellular analysis.
Purpose of the Study:
- To explore and validate the application of mass cytometry for quantifying metal nanoparticle uptake in cells.
- To demonstrate a novel use of ICP-MS beyond traditional cellular heterogeneity studies.
Main Methods:
- Utilized mass cytometry, powered by ICP-MS, to analyze cells in suspension.
- Applied the technique to measure the cellular uptake of metal nanoparticles.
Main Results:
- Successfully quantified metal nanoparticle uptake by cells using mass cytometry.
- Validated the efficacy of ICP-MS for this novel application in cell biology.
Conclusions:
- Mass cytometry, via ICP-MS, offers a robust method for assessing metal nanoparticle internalization in cellular systems.
- This technique expands the utility of mass cytometry for nanoparticle research and toxicology.
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