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Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
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PBRM1 Regulates Stress Response in Epithelial Cells.
Elizabeth G Porter1, Alisha Dhiman1, Basudev Chowdhury1
1Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN 47906, USA.
Iscience
|May 12, 2019
Summary
Polybromo1 (PBRM1) is crucial for epithelial cell maintenance and survival under stress. Loss of PBRM1 increases reactive oxygen species and impairs viability during cellular stress.
Area of Science:
- Molecular Biology
- Cancer Research
- Cell Biology
Background:
- Polybromo1 (PBRM1) is a chromatin remodeler subunit frequently mutated in cancers, especially clear cell renal carcinoma.
- PBRM1 is part of the SWI/SNF chromatin remodeling complex, specifically the PBAF subcomplex, and possesses six tandem bromodomains.
Purpose of the Study:
- To elucidate the role of PBRM1 in epithelial cell maintenance.
- To investigate the impact of PBRM1 loss on cellular responses to stress and apoptosis.
Main Methods:
- Gene expression analysis related to cell adhesion, metabolism, stress response, and apoptosis.
- Assessment of reactive oxygen species generation.
- Evaluation of cellular viability under varying stress conditions.
Main Results:
- PBRM1 regulates genes essential for epithelial cell adhesion, metabolism, stress response, and apoptosis.
- Loss of PBRM1 leads to increased reactive oxygen species production.
- PBRM1 deficiency compromises cellular viability under stress but promotes growth in favorable conditions.
Conclusions:
- PBRM1 plays a significant role in maintaining epithelial cell integrity and function.
- PBRM1 is critical for cellular survival during periods of stress, despite promoting growth under normal conditions.
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