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Related Experiment Videos

Guoliang Sa1, Zhikang Liu2, Jiangang Ren1

  • 1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China; Department of Oral Maxillofacial Surgery, School & Hospital of Stomatology, Wuhan University, Wuhan, China.

Cellular Signalling
|May 15, 2019
PubMed
Summary
This summary is machine-generated.

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Keratinocyte growth factor (KGF) triggers oral epithelial cell adhesion and rete peg elongation by inducing podosome formation. This process involves integrins and Erk1/2 signaling, revealing a new mechanism for epithelial growth.

Area of Science:

  • Cell Biology
  • Oral Epithelial Biology
  • Extracellular Matrix Remodeling

Background:

  • Integrins play a role in keratinocyte growth factor (KGF)-induced oral epithelial adhesion and rete peg elongation.
  • The interplay between extracellular matrix (ECM) remodeling and enhanced epithelial adhesion during rete peg elongation remains unclear.
  • Podosomes are crucial cell-matrix adhesion structures with matrix degradation capabilities.

Purpose of the Study:

  • To investigate the role of podosome formation in KGF-induced oral epithelial cell adhesion and ECM remodeling.
  • To elucidate the signaling pathways, specifically integrin-Erk1/2 signaling, involved in KGF-mediated podosome formation.
  • To understand the contribution of ECM degradation by podosomes to oral epithelial growth.

Main Methods:

Keywords:
ActinCell-matrix junctionCortactinMatrix metalloproteinaseMouth mucosaPodosomes

Related Experiment Videos

  • Treatment of human immortalized oral epithelial cells (HIOECs) with KGF.
  • Identification and characterization of podosome formation using immunofluorescence.
  • Analysis of podosomal components (integrins, MMP14, F-actin, cortactin) and their colocalization.
  • Matrix degradation assays to assess podosome function.
  • Inhibition studies using blocking antibodies, small interfering RNA (siRNA), and Erk1/2 inhibitors.

Main Results:

  • KGF treatment induced podosome formation in HIOECs.
  • Integrin subunits (α6, β4, α3, β1) and MMP14 colocalized with F-actin and cortactin in podosomes.
  • Podosomes exhibited matrix degradation activity.
  • Inhibition of integrin subunits β4, β1, or Erk1/2 blocked KGF-induced podosome formation.
  • Integrin β4 and β1 knockdown reduced Erk1/2 phosphorylation, while Erk1/2 inhibition increased integrin β4 and β1 expression.

Conclusions:

  • KGF induces podosome formation in oral epithelial cells through an integrin-Erk1/2 signaling pathway.
  • This signaling axis is critical for regulating oral epithelial adhesion and rete peg elongation.
  • Podosomes contribute to ECM remodeling, facilitating oral epithelial growth and adhesion.