Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Switching of BJT01:22

Switching of BJT

818
Switching behavior in Bipolar Junction Transistors (BJTs) is a fundamental aspect utilized in various electronic circuits, particularly for digital logic applications like switches and amplifiers. In a typical switching circuit, a BJT alternates between cut-off and saturation modes, corresponding to the "off" and "on" states, respectively, thus behaving like an ideal switch.
Cut-off Mode ("Off" State): In this state, both the emitter-base and collector-base junctions are...
818
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

7.4K
Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
7.4K
Molecular Models02:00

Molecular Models

43.6K
Physical models representing molecular architectures of chemical compounds play essential roles in understanding chemistry. The use of molecular models makes it easier to visualize the structures and shapes of atoms and molecules.
43.6K
Molecular and Ionic Solids02:54

Molecular and Ionic Solids

20.0K
Crystalline solids are divided into four types: molecular, ionic, metallic, and covalent network based on the type of constituent units and their interparticle interactions.
Molecular Solids
Molecular crystalline solids, such as ice, sucrose (table sugar), and iodine, are solids that are composed of neutral molecules as their constituent units. These molecules are held together by weak intermolecular forces such as London dispersion forces, dipole-dipole interactions, or hydrogen bonds, which...
20.0K
Molecular Orbital Theory II03:51

Molecular Orbital Theory II

27.0K
Molecular Orbital Energy Diagrams
27.0K
Molecular Orbital Theory I02:35

Molecular Orbital Theory I

47.1K
Overview of Molecular Orbital Theory
47.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dibenzoylmethane, a novel β-diketone pore blocker of large-conductance calcium-activated potassium channel.

bioRxiv : the preprint server for biology·2026
Same author

CacyBP/SIP - RPL6 interaction: potential influence on ribosome function.

Amino acids·2025
Same author

Cross-variant immune shield: computational multiepitope vaccine design against B.617.2 to Omicron sub-lineages in SARS-CoV-2.

Journal of biomolecular structure & dynamics·2025
Same author

Large scale investigation of GPCR molecular dynamics data uncovers allosteric sites and lateral gateways.

Nature communications·2025
Same author

A bio. tools collection of online resources for GPCR research.

British journal of pharmacology·2025
Same author

GPCR oligomerization across classes: A2AR-mediated regulation of mGlu5R activation.

International journal of biological macromolecules·2025

Related Experiment Video

Updated: Jan 25, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

9.0K

Molecular switches in GPCRs.

Slawomir Filipek1

  • 1Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, ul. Pasteura 1, 02-093 Warsaw, Poland.

Current Opinion in Structural Biology
|May 15, 2019
PubMed
Summary
This summary is machine-generated.

Molecular switches in G protein-coupled receptors (GPCRs) transmit signals by undergoing conformational changes. Agonist binding triggers these switches, facilitating water influx and signal propagation to the cell interior.

More Related Videos

Measuring the Switch Cost of Smartphone Use While Walking
07:00

Measuring the Switch Cost of Smartphone Use While Walking

Published on: April 30, 2020

2.2K
An In Vitro System to Study Tumor Dormancy and the Switch to Metastatic Growth
09:14

An In Vitro System to Study Tumor Dormancy and the Switch to Metastatic Growth

Published on: August 11, 2011

16.3K

Related Experiment Videos

Last Updated: Jan 25, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

9.0K
Measuring the Switch Cost of Smartphone Use While Walking
07:00

Measuring the Switch Cost of Smartphone Use While Walking

Published on: April 30, 2020

2.2K
An In Vitro System to Study Tumor Dormancy and the Switch to Metastatic Growth
09:14

An In Vitro System to Study Tumor Dormancy and the Switch to Metastatic Growth

Published on: August 11, 2011

16.3K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • G protein-coupled receptors (GPCRs) are crucial cell surface receptors involved in signal transduction.
  • Molecular switches within GPCRs are essential for relaying extracellular signals to the intracellular environment.
  • Conserved structural motifs on transmembrane helices are implicated in the function of these molecular switches.

Purpose of the Study:

  • To elucidate the mechanism of molecular switches in GPCRs.
  • To understand how agonist binding initiates signal transmission across the receptor.
  • To investigate the role of water molecules in GPCR activation.

Main Methods:

  • Analysis of conserved structural motifs in GPCR transmembrane helices.
  • Investigating the role of adjacent residues in signal propagation.
  • Studying the conformational changes associated with agonist binding and lock-breaking events.
  • Observing water molecule influx and its role in forming intracellular pathways.

Main Results:

  • Molecular switches, often involving conserved motifs and adjacent residues, are identified within GPCRs.
  • Agonist binding can induce conformational changes, akin to breaking molecular locks, to initiate signaling.
  • A cascade of switch activations correlates with significant water molecule influx.
  • Water molecules facilitate the formation of a continuous pathway across the receptor, aiding signal transduction by disrupting hydrophobic barriers and reorganizing hydrogen bonds.

Conclusions:

  • Molecular switches are key components in GPCR signal transduction, translating agonist binding into intracellular responses.
  • The dynamic interplay of receptor conformation, water molecules, and hydrogen bond networks is critical for GPCR activation.
  • Understanding these mechanisms provides insights into GPCR function and potential drug development targets.