Related Experiment Video
Updated: Jan 24, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
Published on: December 1, 2017
High-throughput Quant-iT PicoGreen assay using an automated liquid handling system
Kay Anantanawat1,2, Nicola Pitsch1, Caroline Fromont1,3
1The Next Generation Sequencing Facility, Western Sydney University, Sydney, Australia.
Abstract:
Workflows in NGS facilities require high-standard practices and high-throughput pipelines to process the large number of samples received in a timely manner. Downstream protocols such as NGS library preparation require accurate estimation of nucleic acid concentrations, which can be achieved using fluorescent dye-based nucleic acid measurement. Here, we report a protocol for preparing a 384-well Quant-iT PicoGreen assay. The protocol allows the concentrations of 184 DNA samples to be measured simultaneously in duplicate in only 1 h using an Eppendorf epMotion 5075 liquid handling system. The advantages of this high-throughput approach include a reduction in both reagents (10x less reagents compared to a standard protocol) and time (3 h for 384 samples compared with 3 days).
More Related Videos
Related Concept Videos
Sample Handling
Samples should be transported carefully from collection points to the laboratory. They should be properly sealed and clearly labeled to prevent cross-contamination. To preserve the sample integrity, optimal temperature conditions during transport are essential. This could involve using...
Molecular Comparison of Gases, Liquids, and Solids
Distribution Reliability and Automation
Rise of Liquid in a Capillary Tube
Deriving the Speed of Sound in a Liquid
The speed of sound in fluids can be derived by considering a mechanical wave...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:

