Hg²⁺-binding peptide decreases mercury ion accumulation in fish through a cell surface display system

Mercury is a potentially toxic trace metal that poses threats to aquatic life and to humans. In this study, a mercury-binding peptide was displayed on the surface of Escherichia coli cells using an N-terminal region ice nucleation protein anchor. The surface-engineered E. coli facilitated selective adsorption of mercury ions (Hg²⁺) from a solution containing various metal ions. The Hg²⁺ adsorption capacity of the surface-engineered cell was four-fold higher than that of the original E. coli cells. Approximately 95% of Hg²⁺ was removed from solution by these whole-cell sorbents. The transformed strains were fed to Carassius auratus, so that the bacteria could colonize fish intestine. Engineered bacteria-fed C. auratus showed significantly less (51.1%) accumulation of total mercury when compared with the group that had not been fed engineered bacteria. The surface-engineered E. coli effectively protected fish against the toxicity of Hg²⁺ in aquatic environments by adsorbing more Hg²⁺. Furthermore, the surface-engineered E. coli mitigated microbial diversity changes in the intestine caused by Hg²⁺ exposure, thereby protecting the intestinal microbial community. This strategy is a novel approach for controlling Hg²⁺ contamination in fish.